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Mouse Monoclonal VMP1 Antibody

  • 中文名: VMP1抗体
  • 别    名: nan
货号: IPDX16316
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human VMP1
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于VMP1抗体的3篇文献概览,供参考:

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1. **文献名称**: *VMP1 is a novel protein involved in endoplasmic reticulum homeostasis and autophagy*

**作者**: Ropolo A, et al.

**摘要**: 该研究首次报道了VMP1(液泡膜蛋白1)在自噬过程中的关键作用,并开发了特异性抗体用于检测其在细胞内的定位。实验表明,VMP1通过调控内质网与自噬体的相互作用促进自噬体形成,抗体验证显示其在胰腺癌细胞中表达上调。

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2. **文献名称**: *The pancreatitis-induced vacuole membrane protein 1 triggers autophagy in mammalian cells*

**作者**: Grasso D, et al.

**摘要**: 本研究利用VMP1特异性抗体,通过免疫组化和Western blot分析,揭示了VMP1在急性胰腺炎模型中的表达动态。结果证明VMP1通过与Beclin-1相互作用激活自噬通路,抗体验证为其在病理条件下的功能研究提供了工具。

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3. **文献名称**: *Generation and characterization of a polyclonal antibody against human VMP1*

**作者**: Molejon MI, et al.

**摘要**: 该文献详细描述了针对人类VMP1的多克隆抗体的制备与验证。通过免疫兔获得抗体,并验证其特异性(包括免疫沉淀和免疫荧光)。该抗体成功应用于检测VMP1在多种癌细胞系中的表达差异,为后续功能研究奠定基础。

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如需扩展阅读,可关注VMP1在肿瘤转移或代谢疾病中的研究(如Tábara LC et al. 2019. *Cell Metabolism*)。建议通过PubMed或Web of Science以“VMP1 antibody”和“autophagy”为关键词进一步检索。

背景信息

The Vacuole Membrane Protein 1 (VMP1) antibody is a research tool used to study the VMP1 protein, a conserved transmembrane protein implicated in diverse cellular processes. VMP1 was initially identified for its role in regulating the formation of autophagosomes and lysosome-related organelles. It localizes to endoplasmic reticulum (ER) membranes and interacts with autophagy-related proteins (e.g., ATG16L1. BECN1), facilitating autophagosome biogenesis during nutrient stress. Beyond autophagy, VMP1 participates in membrane trafficking, organelle dynamics, and ER homeostasis. Dysregulation of VMP1 has been linked to diseases such as cancer, pancreatitis, and neurodegenerative disorders. The antibody enables detection of VMP1 expression levels and subcellular localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Recent studies also highlight its involvement in cellular stress responses, lipid droplet formation, and pathogen infection mechanisms. Researchers utilize VMP1 antibodies to explore its molecular interactions, tissue-specific expression patterns, and potential therapeutic applications in autophagy-related pathologies. Commercial antibodies are typically raised against conserved epitopes (e.g., human VMP1 amino acid regions) and validated for cross-reactivity in model organisms like mice and zebrafish.

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