| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ARHGAP45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ARHGAP45抗体的假设性参考文献示例(请注意，文献名称和作者为虚构案例，建议通过学术数据库核实真实文献)：
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1. **文献名称**：ARHGAP45 regulates T-cell activation through cytoskeletal remodeling
**作者**：Smith A, et al.
**摘要**：研究利用ARHGAP45抗体进行Western blot和免疫荧光实验，发现ARHGAP45通过抑制RhoA信号通路调控T细胞活化过程中的细胞骨架重组，影响免疫突触形成。
2. **文献名称**：Expression profiling of ARHGAP45 in lung cancer using a novel monoclonal antibody
**作者**：Zhang L, et al.
**摘要**：开发了一种特异性ARHGAP45单克隆抗体，并通过免疫组化分析发现其在肺癌组织中高表达，提示其可能作为肿瘤转移的生物标志物。
3. **文献名称**：ARHGAP45 interacts with WAVE2 to modulate dendritic cell migration
**作者**：Johnson R, et al.
**摘要**：通过免疫共沉淀(Co-IP)结合ARHGAP45抗体，揭示了ARHGAP45与WAVE2复合物的相互作用，证明其在树突状细胞趋化运动中的关键作用。
4. **文献名称**：Functional analysis of ARHGAP45 knockout mice using antibody-based validation
**作者**：Lee H, et al.
**摘要**：利用ARHGAP45抗体验证基因敲除小鼠模型，发现ARHGAP45缺失导致巨噬细胞吞噬功能异常，证实其在先天免疫中的功能。
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建议通过PubMed、Google Scholar等平台，以“ARHGAP45 antibody”或“ARHGAP45 function”为关键词检索真实文献。如需具体文献协助，请提供更多研究背景。
The ARHGAP45 (Rho GTPase-activating protein 45) antibody is a tool used to study the function of ARHGAP45. a member of the RhoGAP family that regulates Rho GTPases, particularly Rac and Cdc42. These GTPases are critical for cytoskeletal dynamics, cell migration, and signal transduction. ARHGAP45 acts as a negative regulator by enhancing the intrinsic GTPase activity of Rac/Cdc42. converting them to an inactive GDP-bound state, thereby modulating cellular processes like immune cell activation, neuronal development, and cancer cell invasion.
ARHGAP45 antibodies are widely utilized in immunoblotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous ARHGAP45 expression across tissues, with high specificity confirmed through knockout controls. They help investigate its role in pathways such as T-cell receptor signaling, where ARHGAP45 interacts with proteins like LCP2 and LCK to suppress excessive immune responses. Dysregulation of ARHGAP45 has been linked to autoimmune diseases and malignancies, making these antibodies valuable in translational research.
Commercial ARHGAP45 antibodies are available as monoclonal or polyclonal versions from suppliers like Cell Signaling Technology and Santa Cruz Biotechnology, often validated in human, mouse, or rat samples. Researchers use them to explore tissue-specific expression patterns, post-translational modifications, or interactions in co-immunoprecipitation assays. Proper validation, including peptide blocking and cross-reactivity checks, is essential due to potential isoform diversity (e.g., alternative splicing variants). Recent studies also highlight its involvement in neurodevelopmental disorders, expanding its biomedical relevance. (Word count: 248)
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