| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ARAF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ARAF抗体的参考文献及摘要概述：
1. **文献名称**：*Targeting ARAF mutations in lung cancer: Molecular characterization and drug sensitivity*
**作者**：Smith J, et al.
**摘要**：该研究分析了非小细胞肺癌(NSCLC)中ARAF激酶结构域突变的功能影响，利用特异性ARAF抗体检测突变蛋白表达，发现某些突变导致MEK/ERK信号通路异常激活，并影响肿瘤对RAF抑制剂的敏感性。
2. **文献名称**：*ARAF antibody validation for immunohistochemistry in melanoma diagnosis*
**作者**：Lee S, et al.
**摘要**：研究团队系统验证了商用ARAF抗体在黑色素瘤组织中的特异性，证明其在免疫组化(IHC)中可有效区分ARAF高表达肿瘤与正常组织，为ARAF作为黑色素瘤生物标志物提供技术支持。
3. **文献名称**：*ARAF isoforms in developmental signaling: Insights from antibody-based profiling*
**作者**：Garcia-Ruiz C, et al.
**摘要**：通过开发针对不同ARAF异构体的特异性抗体，揭示了ARAF在胚胎发育阶段MAPK信号通路中的异构体特异性调控机制，为理解ARAF功能多样性提供实验依据。
备注：以上文献为示例，实际文献需通过PubMed或Google Scholar检索关键词“ARAF antibody”、“ARAF kinase detection”获取最新研究。
ARAF (A-Raf proto-oncogene) is a member of the RAF kinase family, which plays a critical role in the MAPK/ERK signaling pathway. This pathway regulates essential cellular processes, including proliferation, differentiation, and survival. ARAF, along with BRAF and CRAF (RAF1), functions as a serine/threonine kinase activated by RAS proteins. Upon activation, RAF kinases phosphorylate MEK1/2. triggering a cascade that ultimately modulates gene expression. While BRAF is the most studied due to its frequent oncogenic mutations in cancers like melanoma, ARAF is less characterized but shares structural similarities with its homologs.
ARAF antibodies are essential tools for studying its expression, localization, and activation in both physiological and pathological contexts. Researchers use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore ARAF's role in diseases, particularly cancers. Although ARAF mutations are rare compared to BRAF, dysregulated ARAF signaling has been implicated in tumorigenesis, drug resistance, and developmental disorders. For example, certain ARAF variants are linked to Noonan syndrome and other RASopathies.
Despite its lower kinase activity relative to BRAF/CRAF, ARAF's unique interactions with regulatory proteins and context-dependent functions make it a subject of ongoing research. ARAF antibodies also aid in evaluating therapeutic strategies targeting the MAPK pathway, such as RAF inhibitors. However, cross-reactivity with other RAF isoforms remains a technical challenge, necessitating antibody validation. Overall, ARAF antibodies contribute to unraveling the complexity of RAF signaling and its implications in health and disease.
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