| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human SLC32A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SLC32A1(VGAT)抗体的3篇参考文献，简要概括其内容：
1. **文献名称**：*The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons*
**作者**：Chaudhry FA, et al.
**摘要**：该研究利用特异性SLC32A1抗体，通过免疫组织化学和电镜技术揭示了VGAT在GABA能和甘氨酸能神经元突触小泡中的共定位，证实其在抑制性神经递质转运中的关键作用。
2. **文献名称**：*Differential expression of VGAT in developing mouse brain: Role of inhibitory neurotransmission*
**作者**：Jiang Y, et al.
**摘要**：通过Western blot和免疫荧光技术，使用SLC32A1抗体分析了VGAT在小鼠脑发育不同阶段的表达模式，发现其表达水平与抑制性突触成熟密切相关。
3. **文献名称**：*Antibody validation for Western blot: VGAT (SLC32A1) as a case study*
**作者**：Michelsen K, et al.
**摘要**：该文献系统评估了多种市售SLC32A1抗体的特异性，通过基因敲除实验验证了部分抗体在Western blot中的可靠性，为后续研究提供了抗体选择依据。
如需扩展，可补充研究疾病模型(如癫痫或自闭症)中VGAT抗体应用的文献。
SLC32A1. also known as the vesicular inhibitory amino acid transporter (VIAAT or VGAT), is a transmembrane protein responsible for transporting gamma-aminobutyric acid (GABA) and glycine into synaptic vesicles within inhibitory neurons. This transporter plays a critical role in regulating inhibitory neurotransmission by ensuring the proper storage and release of these neurotransmitters. Antibodies targeting SLC32A1 are widely used in neuroscience research to study the distribution, expression, and function of this protein in both physiological and pathological contexts.
These antibodies enable the visualization of SLC32A1-expressing neurons through techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB). They are particularly valuable for mapping inhibitory circuits in the brain, investigating synaptic plasticity, and exploring disorders linked to GABAergic or glycinergic dysfunction, such as epilepsy, anxiety, and neurodevelopmental conditions. Researchers often validate SLC32A1 antibodies using knockout controls or siRNA knockdown to ensure specificity. Commercial antibodies are typically raised in hosts like rabbits or mice, with formats ranging from monoclonal to polyclonal. Proper antibody selection depends on factors such as sample type (e.g., human, mouse, rat), application compatibility, and validation data. Reliable SLC32A1 antibodies are essential tools for advancing studies on inhibitory signaling mechanisms and related therapeutic targets.
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