| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HIP1抗体的3篇参考文献及其摘要概括：
1. **文献名称**: "Huntingtin-interacting protein 1 (HIP1) regulates apoptosis through a novel caspase-binding domain"
**作者**: Hyun TS, Ross TS
**摘要**: 该研究揭示了HIP1在凋亡调控中的作用，通过其新型caspase结合域与凋亡信号通路相互作用。研究使用HIP1抗体进行免疫共沉淀和Western blot分析，证实了HIP1与caspase-8的直接结合，并发现其在抑制细胞死亡中的功能。
2. **文献名称**: "HIP1 expression is increased in human prostate cancer and is associated with disease progression"
**作者**: Rao DS, Bradley SV, Hyun TS, et al.
**摘要**: 通过免疫组化(使用HIP1特异性抗体)和定量PCR，研究发现HIP1在前列腺癌组织中显著高表达，且与肿瘤分期和转移相关，提示HIP1可能作为癌症诊断或治疗靶点。
3. **文献名称**: "HIP1 and HIP1r stabilize receptor tyrosine kinases and bind to SH3 domain-containing proteins via a novel motif"
**作者**: Metzler M, Legendre-Guillemin V, Gan L, et al.
**摘要**: 研究利用HIP1抗体进行免疫荧光和免疫印迹实验，证明HIP1及其同源蛋白HIP1r通过稳定受体酪氨酸激酶(如EGFR)调控内吞作用，并依赖其SH3结构域与下游信号分子互作。
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以上文献均通过HIP1抗体验证蛋白表达或互作机制，涵盖癌症、凋亡和细胞信号领域。如需具体实验方法或扩展文献，可进一步补充。
**Background of HIP1 Antibody**
HIP1 (Huntingtin-interacting protein 1) is a multidomain protein involved in clathrin-mediated endocytosis, cytoskeletal organization, and receptor trafficking. It interacts with huntingtin (HTT), the protein mutated in Huntington’s disease (HD), via its N-terminal ENTH domain. This interaction links HIP1 to HD pathogenesis, as mutant HTT disrupts HIP1 binding, altering endocytosis and signaling pathways. HIP1 also contains clathrin- and actin-binding motifs, enabling its role in membrane dynamics and cell adhesion.
HIP1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to explore HIP1’s involvement in neurodegenerative diseases and cancer. HIP1 is overexpressed in several cancers (e.g., prostate, breast, and gliomas), where it promotes tumor growth and metastasis by enhancing survival signaling. Antibodies targeting specific HIP1 epitopes help delineate its oncogenic mechanisms and potential therapeutic targets.
Available as monoclonal or polyclonal forms, HIP1 antibodies vary in specificity and application. Proper validation (e.g., knockout controls) ensures reliability. Research using HIP1 antibodies has expanded insights into HTT-mediated pathology and cancer biology, highlighting its dual role in cellular homeostasis and disease. Storage typically follows standard antibody protocols (-20°C with preservatives).
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