| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | CD104 |
| Entrez GeneID | 3691 |
| clone | 10B10D5 |
| WB Predicted band size | 202kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ITGB4 (AA: 1619-1822) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条关于Rat IgG2a抗体的参考文献示例(内容为虚构，仅供格式参考)：
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1. **标题**: "Characterization of Rat IgG2a Monoclonal Antibodies in Autoimmune Disease Models"
**作者**: Smith A, et al.
**摘要**: 研究比较了不同Rat IgG2a单克隆抗体在小鼠自身免疫模型中的结合特异性和中和活性，揭示了其通过Fc受体介导的免疫调节功能。
2. **标题**: "Optimization of Rat IgG2a as Isotype Controls in Flow Cytometry"
**作者**: Johnson R, et al.
**摘要**: 探讨了Rat IgG2a同型对照抗体在流式细胞术中的标准化应用，验证其低非特异性结合特性，为实验设计提供参考。
3. **标题**: "Structural Stability of Rat IgG2a in Neutralizing Viral Pathogens"
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析Rat IgG2a抗体结构，证明其铰链区柔性对病毒表面抗原的中和效力具有关键作用。
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(注：以上文献为示例，实际引用请通过PubMed或学术数据库查询具体研究。)
Rat IgG2a is a subclass of immunoglobulin G (IgG) antibodies produced in rats, playing a critical role in adaptive immune responses. As one of the four IgG subclasses in rats (IgG1. IgG2a, IgG2b, IgG2c), IgG2a is distinguished by its heavy chain structure, which determines its functional specificity. It is primarily involved in combating pathogens through mechanisms like neutralization, complement activation, and antibody-dependent cellular cytotoxicity (ADCC).
Rat IgG2a antibodies are often utilized in research and diagnostic applications due to their high affinity and specificity. They are commonly employed as secondary antibodies in immunoassays (e.g., ELISA, Western blot, immunohistochemistry) to detect target antigens when paired with primary antibodies from other species. Additionally, they serve as critical controls in experiments to validate assay specificity, ruling out nonspecific binding or background noise.
In therapeutic contexts, rat IgG2a monoclonal antibodies have been engineered for preclinical studies, particularly in cancer immunotherapy and autoimmune disease models. Their Fc region interacts with Fc receptors on immune cells, influencing effector functions. However, differences in Fc receptor binding between species must be considered when translating rodent-derived antibodies to human applications. Advances in recombinant DNA technology have enabled the generation of chimeric or humanized IgG2a variants to reduce immunogenicity in clinical settings. Overall, rat IgG2a remains a cornerstone tool in biomedical research, bridging experimental and translational studies.
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