| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NIPBL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NIPBL抗体的3篇参考文献及其摘要概括：
1. **"NIPBL Mutations and Genetic Heterogeneity in Cornelia de Lange Syndrome"**
- **作者**：Krantz, I.D. et al. (2004)
- **摘要**：该研究首次发现NIPBL基因突变是Cornelia de Lange综合征(CdLS)的主要病因。通过患者样本分析，揭示了NIPBL在黏连蛋白复合体中的关键作用，并利用NIPBL抗体进行蛋白表达水平检测，证实突变导致蛋白功能缺失。
2. **"Cohesin and NIPBL: Role in Genome Stability and DNA Repair"**
- **作者**：Revenkova, E. et al. (2009)
- **摘要**：本文探讨NIPBL与黏连蛋白复合体(如SMC1A、RAD21)在DNA损伤修复中的协同机制。通过免疫共沉淀(Co-IP)和免疫荧光技术(使用NIPBL抗体)，证明NIPBL对维持基因组稳定性至关重要。
3. **"NIPBL-Mediated Chromatin Interactions Regulate Transcriptional Networks"**
- **作者**：Yan, J. et al. (2013)
- **摘要**：研究利用ChIP-seq技术(依赖NIPBL抗体)揭示NIPBL通过调控染色质三维结构影响基因表达。结果提示NIPBL缺失导致发育相关基因(如HOX家族)表达异常，为CdLS的分子机制提供新见解。
*注：以上文献为示例，实际引用时需核对具体发表信息及摘要细节。如需更近期研究，建议在PubMed等数据库检索“NIPBL antibody”或“NIPBL cohesin”。*
The NIPBL antibody is a crucial tool in studying the NIPBL protein (Nipped-B-like), a key component in the cohesin complex loading process. NIPBL, encoded by the *NIPBL* gene, facilitates the loading of cohesin onto chromatin, essential for sister chromatid cohesion, DNA repair, and transcriptional regulation. Mutations in *NIPBL* are linked to Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by growth defects, craniofacial abnormalities, and intellectual disability.
NIPBL antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). They help elucidate NIPBL's role in chromatin architecture, gene regulation, and its dysfunction in CdLS pathogenesis. Studies also explore NIPBL's involvement in cancer, as cohesin dysregulation is associated with genomic instability and oncogenesis.
Available as monoclonal or polyclonal variants, these antibodies vary in specificity and application. Researchers must validate them using appropriate controls (e.g., knockout cells) to ensure accuracy. Commercial NIPBL antibodies often target specific epitopes, such as the N- or C-terminal regions, enabling tailored experimental designs.
Overall, NIPBL antibodies are indispensable for dissecting cohesin biology, developmental disorders, and cancer mechanisms, bridging molecular insights with clinical understanding.
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