Mouse Monoclonal NRBF2 Antibody

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     Lane 1: 15 µg of K562 lysate;  Lane 2: 15 µg of Jurkat lysate;  Lane 3: 15 µg of HepG2 lysate;Lane 4: 15 µg of HeLa lysate;  Result: P16076 can detect NRBF2 by Western blotting.    

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     Lane 1: Hep G2 lysate;  Lane 2: NRBF2 immunoprecipitated from Hep G2 lysate by P16076;  Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P16076 can immunoprecipitate NRBF2;    

The NRBF2 (Nuclear Receptor Binding Factor 2) antibody is a tool used to study the function and regulation of the NRBF2 protein, a critical component of the phosphatidylinositol 3-kinase class III (PI3K-III) complex. NRBF2. also known as COAT or Parc, acts as a regulatory subunit that stabilizes the PI3K-III complex, which is essential for autophagy, endosomal trafficking, and cytokinesis. It modulates the enzymatic activity of VPS34 (vacuolar protein sorting 34), the catalytic subunit of the complex, thereby influencing lipid kinase signaling and membrane dynamics. Dysregulation of NRBF2 has been linked to neurodegenerative diseases, cancer, and metabolic disorders, making it a focus of pathophysiological research.

The NRBF2 antibody enables the detection and quantification of NRBF2 protein levels in various experimental models, including Western blotting, immunofluorescence, and immunohistochemistry. Researchers use it to investigate NRBF2's subcellular localization, interaction partners, and expression patterns under different physiological or stress conditions. Its specificity is crucial for distinguishing NRBF2 isoforms or post-translational modifications. Studies employing this antibody have revealed NRBF2's dual role in autophagy—both promoting and inhibiting processes depending on cellular context—highlighting its complex regulatory mechanisms. As autophagy-targeted therapies gain traction, the NRBF2 antibody remains vital for elucidating molecular pathways and validating therapeutic targets in disease models.