| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human MAPRE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MAPRE3抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过数据库核实)：
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1. **文献名称**：*MAPRE3 modulates microtubule dynamics and promotes glioblastoma cell invasion*
**作者**：Chen L, et al.
**摘要**：本研究利用MAPRE3特异性抗体通过免疫印迹和免疫荧光技术，发现MAPRE3在胶质母细胞瘤中高表达，并通过调控微管稳定性促进肿瘤细胞侵袭。抗体验证显示其在患者组织样本中的定位与临床预后相关。
2. **文献名称**：*EB3 (MAPRE3) regulates neuronal microtubule polarity and axonal transport*
**作者**：Fukata Y, et al.
**摘要**：通过MAPRE3抗体进行免疫沉淀和活细胞成像，作者发现MAPRE3与微管正端结合蛋白EB1协同作用，调控神经元轴突运输的定向性，为神经退行性疾病机制提供了新见解。
3. **文献名称**：*Differential expression of MAPRE3 in colorectal cancer progression*
**作者**：Wang X, et al.
**摘要**：该研究使用MAPRE3抗体对结直肠癌组织进行免疫组化分析，发现MAPRE3表达水平与肿瘤分期及转移显著相关，提示其作为潜在生物标志物的价值。
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**说明**：
- 以上文献聚焦MAPRE3抗体在疾病机制(如癌症、神经疾病)研究中的应用，涵盖技术方法(Western blot、免疫荧光、免疫组化)。
- 实际检索建议使用PubMed/Google Scholar，以“MAPRE3 antibody”及“应用场景(如cancer, microtubule)”为关键词筛选。
MAPRE3 (Microtubule-Associated Protein RP/EB Family Member 3), also known as EB3. is a member of the End-binding (EB) protein family that plays a critical role in regulating microtubule dynamics. These proteins bind to the growing plus-ends of microtubules, stabilizing their structure and facilitating interactions with cellular components involved in cell division, intracellular transport, and cell polarity. EB3 specifically contributes to microtubule tip-tracking through its calponin homology domain and coordinates with other +TIPs (microtubule plus-end tracking proteins) to regulate cytoskeletal reorganization during processes like neuronal development, cell migration, and vesicle trafficking.
Antibodies targeting MAPRE3/EB3 are essential tools for studying its expression, localization, and function in cellular models. They are widely used in techniques such as immunofluorescence, Western blotting, and immunoprecipitation to visualize microtubule dynamics, investigate EB3’s role in cancer metastasis (where dysregulated microtubule activity is common), or explore its involvement in neurological disorders like Alzheimer’s disease. Some antibodies distinguish EB3 from other EB family members (e.g., EB1. EB2), enabling isoform-specific studies. Researchers must validate these antibodies for cross-reactivity and optimal performance across species (human, mouse, rat) and sample types. Commercial antibodies often provide data on applications and species specificity, but context-dependent validation remains critical for experimental accuracy.
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