| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINB8 |
| Uniprot No | P50452 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDDLCEA NGTFAISLFK ILGEEDNSRN VFFSPMSISS ALAMVFMGAK GSTAAQMSQA LCLYKDGDIH RGFQSLLSEV NRTGTQYLLR TANRLFGEKT CDFLPDFKEY CQKFYQAELE ELSFAEDTEE CRKHINDWVA EKTEGKISEV LDAGTVDPLT KLVLVNAIYF KGKWNEQFDR KYTRGMLFKT NEEKKTVQMM FKEAKFKMGY ADEVHTQVLE LPYVEEELSM VILLPDDNTD LAVVEKALTY EKFKAWTNSE KLTKSKVQVF LPRLKLEESY DLEPFLRRLG MIDAFDEAKA DFSGMSTEKN VPLSKVAHKC FVEVNEEGTE AAAATAVVRN SRCSRMEPRF CADHPFLFFI RHHKTNCILF CGRFSSP |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINB8重组蛋白的3篇代表性文献示例(注：文献信息为示例性质，可能需要根据实际研究调整)：
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1. **文献名称**: "Recombinant SERPINB8 inhibits cathepsin L and epidermal fragility in vitro"
**作者**: Rousseau, A. et al.
**摘要**: 本研究通过大肠杆菌表达系统纯化重组SERPINB8蛋白，发现其能特异性抑制组织蛋白酶L(cathepsin L)的活性，并证明其在维持表皮细胞完整性中起关键作用，为皮肤屏障功能障碍疾病提供分子机制依据。
2. **文献名称**: "SERPINB8 modulates neuronal apoptosis through caspase-3 interaction"
**作者**: Zhang, Y. et al.
**摘要**: 利用HEK293细胞表达重组SERPINB8蛋白，揭示其通过直接结合并抑制caspase-3活性调控神经元凋亡通路，提示其在神经退行性疾病中的潜在治疗价值。
3. **文献名称**: "Structural analysis of SERPINB8-protease complexes by cryo-EM"
**作者**: Kaiser, M. et al.
**摘要**: 通过冷冻电镜解析重组SERPINB8与靶标蛋白酶复合物的三维结构，阐明其抑制机制中的关键构象变化，为设计靶向丝氨酸蛋白酶抑制剂提供结构基础。
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**备注**：以上内容为学术示例，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词“SERPINB8 recombinant protein”或结合具体研究领域(如皮肤生物学、细胞凋亡)筛选近期论文。
**Background of SERPINB8 Recombinant Protein**
SERPINB8. a member of the serine protease inhibitor (SERPIN) superfamily, plays critical roles in regulating proteolytic pathways involved in cellular homeostasis, differentiation, and apoptosis. Specifically, SERPINB8 is classified within the clade B subfamily, which is characterized by intracellular localization and tissue-specific expression patterns. It is predominantly expressed in epithelial tissues, including the skin, and has been implicated in maintaining epidermal barrier function and modulating inflammatory responses.
The recombinant form of SERPINB8 is engineered to mimic the native protein’s structure and function, typically produced using expression systems like *E. coli* or mammalian cell cultures. This allows for high-purity, scalable production while retaining its inhibitory activity against target proteases, such as trypsin-like serine proteases. Studies suggest SERPINB8 interacts with proteases involved in extracellular matrix remodeling and immune signaling, potentially influencing pathologies like skin disorders, cancer metastasis, or neurodegenerative diseases.
Research on SERPINB8 recombinant protein has focused on elucidating its mechanism of protease inhibition, which involves a conserved reactive center loop (RCL) that traps target enzymes in a stable complex. Structural analyses reveal conformational changes critical for its inhibitory function. Additionally, its role in autophagy and stress responses has garnered interest, linking dysregulation of SERPINB8 to diseases like psoriasis or squamous cell carcinoma.
The development of recombinant SERPINB8 facilitates functional studies, drug screening, and therapeutic exploration, though challenges remain in optimizing its stability and specificity for clinical applications. Ongoing research aims to clarify its tissue-specific roles and potential as a biomarker or therapeutic target.
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