Mouse Monoclonal MINDY4 Antibody

The MINDY4 antibody is a research tool developed to study the MINDY4 protein, a member of the MINDY (Motif Interacting with Ubiquitin-containing Novel DUB Family) class of deubiquitinating enzymes (DUBs). MINDY4. also known as FAM63B, is part of a conserved eukaryotic enzyme family that regulates protein degradation by selectively cleaving ubiquitin chains, particularly K48-linked polyubiquitin—a signal for proteasomal destruction. Unlike other DUB families, MINDY enzymes exhibit unique substrate preferences and structural features, including a conserved catalytic domain and a ubiquitin-binding motif. MINDY4 is implicated in cellular processes like protein homeostasis, cell cycle regulation, and DNA damage response, though its precise biological roles remain under investigation. The antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect MINDY4 expression, localization, and interactions in cell lines or tissues. Its development has been driven by interest in DUBs as potential therapeutic targets, given their dysregulation in cancers and neurodegenerative diseases. Validation typically includes specificity tests in MINDY4-knockout models and cross-reactivity assessments against other MINDY paralogs (MINDY1-3). Research using this antibody aims to clarify MINDY4's physiological functions and its potential links to disease mechanisms.