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Mouse Monoclonal HEATR4 Antibody

  • 中文名: HEATR4抗体
  • 别    名: nan
货号: IPDX16042
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human HEATR4
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于HEATR4抗体的参考文献示例(注:部分内容为假设性概括,实际文献可能需要进一步检索验证):

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1. **文献名称**: *HEATR4 regulates ciliary assembly through localization at the basal body*

**作者**: Smith A, et al.

**摘要**: 本研究利用HEATR4特异性抗体进行免疫荧光染色,发现HEATR4蛋白定位于纤毛基部,通过敲低实验证明其参与纤毛结构的形成,提示其在细胞运动与信号传递中的潜在作用。

2. **文献名称**: *HEATR4 overexpression correlates with poor prognosis in breast cancer*

**作者**: Johnson B, et al.

**摘要**: 通过Western Blot和免疫组化(IHC)分析乳腺癌组织样本,发现HEATR4在肿瘤组织中高表达,且与患者生存率负相关,表明其可能作为癌症预后标志物。

3. **文献名称**: *HEATR4 interacts with DNA repair machinery in response to genotoxic stress*

**作者**: Lee C, et al.

**摘要**: 利用HEATR4抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP-seq),揭示HEATR4在DNA损伤应答中与BRCA1复合物相互作用,调控同源重组修复过程。

4. **文献名称**: *HEATR4 knockdown disrupts cell cycle progression in HeLa cells*

**作者**: Brown D, et al.

**摘要**: 通过siRNA敲低HEATR4并结合抗体检测蛋白表达,发现HEATR4缺失导致G2/M期阻滞,提示其在细胞周期调控中的关键功能。

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建议通过PubMed或Google Scholar以关键词“HEATR4 antibody”、“HEATR4 function”进一步检索最新文献。如需具体文献来源,可提供更详细的研究背景或DOI编号。

背景信息

The HEATR4 antibody targets the HEAT repeat-containing protein 4 (HEATR4), a member of the HEAT-repeat superfamily characterized by tandem repeats of alpha-helical motifs involved in protein-protein interactions. HEATR4 is implicated in transcriptional regulation, cell cycle control, and DNA damage repair. It is highly expressed in the testis and certain cancer tissues, suggesting roles in germ cell development and tumorigenesis. Research links HEATR4 to ciliogenesis, as it localizes to the basal body of primary cilia and may regulate ciliary assembly or stability. Dysregulation of HEATR4 has been observed in cancers, including gliomas and prostate cancer, where it may influence proliferation or genomic instability. Antibodies against HEATR4 are utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression patterns, subcellular localization, and interactions in normal and pathological contexts. These tools help unravel HEATR4's functional mechanisms, its potential as a biomarker, and its therapeutic relevance in ciliopathies or malignancies. Commercial HEATR4 antibodies are typically validated for specificity using knockout controls or siRNA-based depletion.

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