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Human heparan sulfate,HS ELISA Kit

  • 中文名： 人硫酸类肝素(HS)ELISA Kit
货号: IPD-E09585h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人硫酸类肝素(HS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的HS含量。人硫酸类肝素（HS）是一种复杂的糖胺聚糖，在细胞外基质中发挥重要作用。其研究机制涉及HS与多种生长因子和细胞表面受体的相互作用，以及其在组织生长、发育和疾病过程中的功能。试剂盒检测范围为31.25 ng/mL-8000 ng/mL，科研人员可利用探索HS在肿瘤微环境调控、代谢性疾病机制或神经退行性疾病中的作用，例如通过检测尿液样本评估肾脏疾病相关HS代谢异常，或分析组织匀浆中HS表达与肿瘤侵袭性的关联，为疾病机制研究和生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    HS
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    31.25 ng/mL-8000 ng/mL
  • 灵敏度：
    31.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human HS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-90


    1:2

    Average %

    88


    Range %

    82-95


    1:4

    Average %

    87


    Range %

    83-93


    1:8

    Average %

    90


    Range %

    83-98


  • 回收率：

    The recovery of human HS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-96


    EDTA plasma (n=4)

    87

    81-94


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average




    8000

    0.313

    0.324

    0.318




    2000

    0.881

    0.847

    0.864




    500

    1.482

    1.528

    1.505




    125

    1.703

    1.739

    1.721




    31.25

    1.977

    2.021

    1.999




    0

    2.241

    2.253

    2.247




  • 本试剂盒所含材料：



    • A 96-well Assay plate --The 96-well plate has been pre-coated with HS.

    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.

    • Anti-human HS Antibody (100 x concentrate) (1 x 60 μl)

    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1

    • HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.

    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.

    • An incubator that can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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