| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ZNF174 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与ZNF174抗体相关的参考文献(基于模拟数据，实际文献需核实)：
1. **文献名称**：*ZNF174 as a DNA methylation-driven suppressor in colorectal cancer*
**作者**：Huang D, et al.
**摘要**：研究通过ZNF174抗体检测发现，ZNF174在结直肠癌组织中因启动子甲基化而表达下调，其缺失促进肿瘤细胞增殖和侵袭，提示其作为抑癌基因的潜在作用。
2. **文献名称**：*Characterization of ZNF174-protein interactions using co-immunoprecipitation*
**作者**：Smith J, et al.
**摘要**：利用ZNF174抗体进行免疫共沉淀实验，鉴定出ZNF174与组蛋白去乙酰化酶(HDACs)的相互作用，表明其可能参与染色质重塑及转录调控通路。
3. **文献名称**：*Tissue-specific expression profiling of ZNF174 in human normal and cancer tissues*
**作者**：Lee S, et al.
**摘要**：通过ZNF174抗体的免疫组化分析，揭示了该蛋白在正常组织(如脑、肾脏)中的高表达，而在多种癌症中表达显著降低，提示其组织特异性调控机制。
注：以上为模拟生成的示例，实际文献需通过PubMed、Google Scholar等平台以“ZNF174 antibody”或“ZNF174 function”为关键词检索。
The zinc finger protein 174 (ZNF174), also known as ZNF306 or ZKSCAN4. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs that bind DNA, enabling sequence-specific transcriptional regulation. ZNF174 is implicated in diverse cellular processes, including chromatin remodeling, cell cycle control, and tumor suppression. Studies suggest its potential role in repressing gene expression by recruiting chromatin-modifying complexes through its KRAB domain.
ZNF174 antibodies are immunological tools designed to detect and study this protein in experimental settings. These antibodies are typically developed in host species like rabbits or mice using immunogenic epitopes derived from ZNF174's unique regions, such as its N-terminal KRAB domain or C-terminal zinc finger clusters. They are widely used in techniques including Western blotting, immunofluorescence, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to investigate ZNF174's expression patterns, subcellular localization (primarily nuclear), and molecular interactions.
Commercially available ZNF174 antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Research utilizing these antibodies has linked ZNF174 to cancer biology, particularly in hepatocellular carcinoma and colorectal cancer, where it may act as a tumor suppressor by modulating pathways like p53 signaling. However, the functional landscape of ZNF174 remains incompletely mapped, driving continued demand for reliable antibodies to explore its physiological and pathological roles.
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