| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MACROH2A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于MACROH2A2抗体的3篇参考文献，包含文献名称、作者和简要摘要内容：
---
1. **文献名称**：*"MacroH2A2 regulates the senescence-associated secretory phenotype"*
**作者**：Smith A, et al.
**摘要**：本研究揭示了MACROH2A2通过调控染色质重塑影响细胞衰老相关分泌表型(SASP)。通过特异性抗体进行ChIP-seq分析，发现MACROH2A2富集在SASP基因启动子区，抑制其表达，为衰老相关疾病提供潜在靶点。
2. **文献名称**：*"Differential expression of macroH2A2 variants in cancer progression"*
**作者**：Chen L, et al.
**摘要**：利用MACROH2A2特异性抗体进行免疫组化分析，发现其表达在乳腺癌组织中显著下调，且与患者预后不良相关。研究提示MACROH2A2可能作为肿瘤抑制因子参与癌症转移调控。
3. **文献名称**：*"MacroH2A2 maintains pluripotency by inhibiting TET-mediated DNA demethylation"*
**作者**：Yamaguchi H, et al.
**摘要**：通过Western blot和免疫荧光实验，证实MACROH2A2抗体可特异性识别多能干细胞中的该组蛋白变体。研究发现MACROH2A2通过拮抗TET酶活性维持干细胞多能性，为表观遗传调控机制提供新见解。
---
注：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“MACROH2A2 antibody”或“macroH2A2 function”检索最新论文。
The MACROH2A2 antibody is a research tool used to detect the macroH2A2 histone variant, a member of the macroH2A family (macroH2A1 and macroH2A2). These variants are characterized by a large C-terminal macrodomain appended to a core histone H2A structure. MacroH2A2 is involved in chromatin remodeling and gene regulation, playing roles in X-chromosome inactivation, cellular differentiation, senescence, and tumor suppression. Unlike macroH2A1. which has two splice isoforms, macroH2A2 exists as a single isoform and exhibits distinct expression patterns, often enriched in repressive chromatin regions associated with transcriptional silencing.
Antibodies targeting macroH2A2 enable researchers to study its localization, expression levels, and functional interactions in epigenetic processes. They are critical for techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in investigations of its role in diseases, particularly cancer. MacroH2A2 has been implicated in suppressing tumor metastasis and maintaining genomic stability, with reduced expression observed in certain malignancies. Its dynamic regulation in response to DNA damage or metabolic signals further highlights its importance in cellular stress responses. However, overlapping structural features between macroH2A1 and macroH2A2 necessitate antibody specificity validation to avoid cross-reactivity. Studies using these antibodies contribute to understanding how chromatin plasticity and histone variants influence cell fate decisions and disease progression, offering potential insights for therapeutic strategies targeting epigenetic dysregulation.
×
