| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD2AP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CD2AP抗体的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
1. **《CD2AP regulates podocyte function and modulates glomerular disease progression》**
- 作者：Shih NY, et al.
- 摘要：通过CD2AP抗体检测，研究发现CD2AP缺陷小鼠出现蛋白尿和肾小球损伤，揭示了CD2AP在维持足细胞结构和滤过屏障中的关键作用，与局灶性节段性肾小球硬化(FSGS)相关。
2. **《CD2AP interacts with the cytoskeleton in T-cell signaling》**
- 作者：Dustin ML, et al.
- 摘要：利用CD2AP抗体进行免疫共沉淀实验，发现CD2AP与CD2受体及肌动蛋白结合，参与免疫突触的形成，影响T细胞活化信号传导。
3. **《CD2AP deficiency enhances amyloid-β pathology in Alzheimer's disease models》**
- 作者：Ubelmann F, et al.
- 摘要：通过CD2AP抗体标记脑组织样本，研究显示CD2AP缺失会加剧淀粉样蛋白沉积，提示其在神经元内吞途径和阿尔茨海默病病理中的调控作用。
4. **《CD2AP as a biomarker for early-stage chronic kidney disease》**
- 作者：Kirsch KH, et al.
- 摘要：采用CD2AP抗体检测患者尿液外泌体中的蛋白表达，发现CD2AP水平与肾功能损伤程度相关，或可作为肾病早期诊断的生物标志物。
(注：上述文献为示例，实际引用需核实真实论文。)
CD2AP (CD2-associated protein) is a cytosolic adaptor protein first identified in 1998 through its interaction with CD2. a cell surface receptor critical for T-cell and natural killer (NK) cell adhesion and immune synapse formation. Primarily expressed in podocytes of the kidney glomerulus, CD2AP plays a key role in maintaining the structural integrity of the slit diaphragm, a specialized cell-cell junction essential for renal filtration. Its scaffolding function facilitates interactions with actin cytoskeleton regulators, cell adhesion molecules (e.g., nephrin), and signaling proteins, enabling dynamic membrane remodeling and signal transduction.
Mutations in the CD2AP gene are linked to focal segmental glomerulosclerosis (FSGS), a condition characterized by podocyte injury and progressive kidney dysfunction. Beyond renal physiology, CD2AP participates in endocytosis, vesicle trafficking, and receptor tyrosine kinase signaling (e.g., PDGFR, VEGFR). Notably, CD2AP has been implicated in neurodegenerative diseases, including Alzheimer’s, through its interaction with proteins involved in amyloid precursor protein (APP) processing.
CD2AP antibodies are widely used in research to study protein localization, expression levels, and molecular interactions via techniques like Western blot, immunofluorescence, and co-immunoprecipitation. These tools have been instrumental in elucidating CD2AP’s dual roles in cellular homeostasis and disease pathogenesis, particularly in nephrology and neurobiology. Commercial antibodies often target specific epitopes within its N-terminal coiled-coil or C-terminal SH3 domains, enabling functional studies across diverse experimental models.
×
