Mouse Monoclonal NT5C1A Antibody

The NT5C1A antibody is a crucial tool for studying the cytosolic 5'-nucleotidase IA (NT5C1A), an enzyme encoded by the *NT5C1A* gene. This protein belongs to the 5'-nucleotidase family, which catalyzes the dephosphorylation of nucleoside monophosphates, such as inosine monophosphate (IMP) and guanosine monophosphate (GMP), to their corresponding nucleosides. NT5C1A plays a role in purine and pyrimidine metabolism, influencing nucleotide homeostasis and cellular signaling pathways. Dysregulation of NT5C1A has been implicated in various cancers, particularly hematologic malignancies like acute lymphoblastic leukemia (ALL), where activating mutations in *NT5C1A* are linked to drug resistance and relapse. These mutations enhance enzymatic activity, promoting the degradation of antimetabolite chemotherapeutics (e.g., 6-thioguanine), thereby reducing treatment efficacy. NT5C1A antibodies are widely used in research to detect protein expression, localization, and function via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They aid in exploring NT5C1A's role in cancer progression, metabolic disorders, and potential therapeutic targeting. Validated antibodies are essential for distinguishing wild-type from mutant forms and assessing clinical correlations. Recent studies highlight NT5C1A's emerging significance as a biomarker and its potential as a target for overcoming chemotherapy resistance in relapsed cancers.