| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDP1抗体的3篇参考文献的简要概括：
1. **文献名称**：*"Characterization of Pyruvate Dehydrogenase Phosphatase 1 (PDP1) Antibody for Mitochondrial Enzyme Activity Studies"*
**作者**：Johnson et al. (2018)
**摘要**：研究开发了一种特异性PDP1抗体，验证其在Western blot和免疫荧光中的应用，揭示了PDP1在线粒体能量代谢中的调控作用，尤其在胰岛素抵抗模型中的表达变化。
2. **文献名称**：*"Role of PDP1 in Cancer Metabolism: Insights from Antibody-Based Proteomic Analysis"*
**作者**：Chen & Lee (2020)
**摘要**：利用商业化PDP1抗体分析多种癌细胞系，发现PDP1表达与糖酵解活性呈负相关，提示其在肿瘤代谢重编程中的潜在靶点价值。
3. **文献名称**：*"Tissue-Specific Distribution of PDP1 Isoforms Revealed by a Novel Polyclonal Antibody"*
**作者**：Martinez et al. (2016)
**摘要**：报道了一种新型多克隆PDP1抗体的开发，通过免疫组化证实PDP1在心脏、肝脏中的高表达，并发现其异构体在不同组织中的功能差异。
(注：以上文献为虚拟示例，实际引用需检索PubMed等数据库获取真实信息。)
The PDP1 antibody is a research tool designed to detect pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1), a protein critical in regulating cellular energy metabolism. PDP1 is a mitochondrial enzyme that activates the pyruvate dehydrogenase complex (PDC) by dephosphorylating its E1 subunit, enabling PDC to convert pyruvate into acetyl-CoA—a key step linking glycolysis to the tricarboxylic acid (TCA) cycle. This regulation is vital for maintaining glucose homeostasis and ATP production.
PDP1 dysfunction is implicated in metabolic disorders, including primary PDP1 deficiency, which disrupts PDC activity and causes lactic acidosis, neurological impairments, and Leigh syndrome. Antibodies targeting PDP1 are essential for studying its expression, localization, and interactions in tissues, particularly in models of diabetes, cancer, and neurodegenerative diseases where mitochondrial metabolism is altered.
These antibodies (often raised in rabbits or mice) are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use PDP1-specific antibodies to explore metabolic adaptations in diseases, screen for therapeutic targets, or diagnose genetic PDP1-related disorders. Recent studies also investigate PDP1's role in cancer progression, as altered PDC activity influences tumor metabolism. By enabling precise detection of PDP1 levels and activity, these antibodies contribute to advancing understanding of mitochondrial regulation and metabolic disease mechanisms.
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