| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HERC5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HERC5抗体的3篇文献示例(内容基于公开研究整理，非真实文献)：
1. **文献名称**：HERC5 regulates ISGylation and influenza A virus resistance through E3 ligase activity
**作者**：Smith J, et al.
**摘要**：研究利用HERC5特异性抗体验证其在流感病毒感染中通过ISGylation修饰宿主蛋白的机制，证明HERC5缺失会增强病毒复制。
2. **文献名称**：HERC5 inhibits HIV-1 replication by promoting ubiquitination of viral Gag protein
**作者**：Li Y, et al.
**摘要**：通过Western blot和免疫共沉淀(使用HERC5抗体)发现HERC5通过泛素化HIV-1 Gag蛋白限制病毒组装，依赖其E3连接酶功能。
3. **文献名称**：HERC5 expression correlates with hepatocellular carcinoma progression and immune response
**作者**：Wang X, et al.
**摘要**：使用HERC5抗体进行免疫组化分析，发现HERC5在肝癌组织中高表达，并与干扰素信号通路激活及患者预后相关。
(注：以上为模拟摘要，实际文献需通过PubMed/Google Scholar等平台以"HERC5 antibody"为关键词检索。)
HERC5 (HECT and RLD domain containing E3 ubiquitin protein ligase 5) is a member of the HERC family of ubiquitin ligases, which play critical roles in protein post-translational modification. Primarily studied in humans, HERC5 is unique for its dual functionality: it acts as an E3 ubiquitin ligase and mediates ISG15 (interferon-stimulated gene 15) conjugation, a process termed ISGylation. This enzyme is strongly induced by type I interferons during viral infections, linking it to innate immune responses. HERC5 targets viral and host proteins for ISGylation, which can disrupt viral replication or modulate immune signaling pathways.
HERC5 antibodies are essential tools for investigating its expression, localization, and interactions in immune regulation, antiviral defense, and cancer. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study HERC5's role in stress responses, cell cycle regulation, and its potential tumor-suppressive or oncogenic effects in various cancers. Dysregulation of HERC5 has been implicated in diseases such as hepatitis, autoimmune disorders, and malignancies, making its study clinically relevant. Antibody specificity is critical due to structural similarities among HERC family members. Recent studies also explore HERC5's involvement in mRNA translation regulation during interferon responses, highlighting its multifaceted roles in cellular physiology.
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