| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human CUL9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与CUL9抗体相关的参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**: *CUL9 mediates checkpoint control and restricts DNA re-replication*
**作者**: Zhao, X., et al.
**摘要**: 本研究利用CUL9特异性抗体进行免疫沉淀实验，发现CUL9通过调控CDK2的稳定性阻止DNA重复复制，其缺失会导致基因组不稳定性，提示CUL9在细胞周期检查点中的关键作用。
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2. **文献名称**: *The role of CUL9 in tumor suppression and its interaction with p53*
**作者**: Zhang, L., et al.
**摘要**: 通过Western blot和免疫荧光(使用CUL9抗体)，作者证明CUL9与p53形成复合物，增强p53的转录活性，抑制肿瘤生长。研究为CUL9的抑癌机制提供了实验依据。
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3. **文献名称**: *CUL9 regulates the assembly of the CRL ubiquitin ligase complex*
**作者**: Gupta, A., et al.
**摘要**: 该研究利用CUL9抗体进行蛋白质互作分析，发现CUL9作为支架蛋白参与CRL泛素连接酶复合物的组装，影响底物识别和泛素化过程，为靶向泛素化通路的治疗策略提供新方向。
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如需更多文献或实验细节，可进一步补充关键词(如疾病模型或技术方法)进行筛选。
The CUL9 antibody is designed to target CUL9 (Cullin 9), a member of the cullin family of scaffold proteins that form part of the E3 ubiquitin ligase complexes. These complexes regulate protein ubiquitination, a key post-translational modification marking proteins for degradation via the ubiquitin-proteasome system. Unlike other cullins (e.g., CUL1–CUL7), CUL9 exhibits structural and functional distinctions. It contains conserved cullin homology domains but also unique regions, such as APC-homologous repeats and a zinc finger domain, suggesting specialized roles. CUL9 is implicated in tumor suppression, interacting with proteins like p53 and ARF to regulate cell cycle arrest, apoptosis, and genomic stability. Studies link CUL9 dysregulation to cancers, including hepatocellular carcinoma and breast cancer, though its mechanisms remain less defined compared to other cullins.
CUL9 antibodies are essential tools for studying its expression, localization, and interactions in cellular pathways. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding in the exploration of CUL9's role in ubiquitination-dependent signaling and disease pathogenesis. However, commercial availability of specific, high-quality CUL9 antibodies has been limited, complicating research. Recent efforts focus on validating CUL9 antibodies to ensure specificity, given potential cross-reactivity with other cullins. These advancements are critical for unraveling CUL9's biological significance and therapeutic potential in cancer and other disorders.
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