| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GLI2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GLI2抗体的3篇参考文献示例(基于公开信息概括，非真实文献，仅供参考格式)：
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1. **文献名称**: "Characterization of GLI2-Specific Antibodies for Hedgehog Signaling Pathway Analysis"
**作者**: Hui CC, et al.
**摘要**: 本研究验证了多种GLI2抗体的特异性，通过Western blot和免疫组化证明其在哺乳动物细胞和组织中的有效性，并发现某些抗体可区分GLI2全长蛋白及其剪切形式，为Hedgehog通路功能研究提供工具。
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2. **文献名称**: "GLI2 Antibody Localization in Embryonic Development and Cancer Models"
**作者**: Ruat M, Briscoe J.
**摘要**: 利用高特异性GLI2抗体，研究者揭示了GLI2在小鼠胚胎神经管和肢芽发育中的动态表达模式，并证明其在基底细胞癌中异常激活，提示抗体在发育生物学和肿瘤研究中的应用潜力。
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3. **文献名称**: "Comparative Analysis of Commercial GLI2 Antibodies for ChIP-seq and Immunoprecipitation"
**作者**: Carpenter RL, Skoda SM.
**摘要**: 通过对比5种市售GLI2抗体的性能，发现两种单克隆抗体在染色质免疫共沉淀(ChIP-seq)中表现最佳，可特异性富集GLI2结合的DNA靶点，为表观遗传学研究提供可靠工具。
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注：以上文献为示例，实际引用需检索PubMed或学术数据库获取真实文献(可搜索关键词：GLI2 antibody, Hedgehog signaling, immunohistochemistry)。
The GLI2 antibody is a crucial tool in studying the Hedgehog (HH) signaling pathway, which plays a pivotal role in embryonic development, tissue homeostasis, and cancer. GLI2. a member of the GLI family of zinc-finger transcription factors (GLI1-3), acts as a primary effector of HH signaling. Upon pathway activation, GLI2 translocates to the nucleus to regulate target gene expression, driving processes like cell proliferation and differentiation. Dysregulation of GLI2 is linked to developmental disorders and cancers, including basal cell carcinoma and medulloblastoma.
GLI2 antibodies are widely used in research to detect and quantify GLI2 protein levels, assess its subcellular localization, and study post-translational modifications. They are essential in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity and validation are critical, as GLI proteins share structural homology, requiring antibodies to distinguish between isoforms. Recent studies also explore GLI2's non-canonical roles beyond HH signaling, such as interactions with other pathways (e.g., TGF-β, Wnt), highlighting its therapeutic relevance. Researchers increasingly utilize GLI2 antibodies to investigate its potential as a biomarker or therapeutic target in HH-driven malignancies.
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