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Mouse Monoclonal CBLL2 Antibody

  • 中文名: CBLL2抗体
  • 别    名: nan
货号: IPDX15618
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human CBLL2
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于CBLL2(Hakai)抗体的参考文献示例,基于现有研究整理:

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1. **文献名称**: *"Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex"*

**作者**: Fujita, Y., et al.

**摘要**: 该研究首次发现Hakai(CBLL2)作为E-cadherin的泛素连接酶,通过泛素化作用促进其内吞和降解。作者使用抗Hakai抗体进行免疫共沉淀和免疫荧光实验,揭示了Hakai在上皮-间质转化(EMT)中的关键作用。

2. **文献名称**: *"Hakai reduces E-cadherin cell surface expression and promotes EMT and metastasis"*

**作者**: Sánchez-Tilló, E., et al.

**摘要**: 研究探讨Hakai(CBLL2)在肿瘤转移中的作用,利用Hakai特异性抗体进行免疫组化和Western blot分析,证明其通过下调E-cadherin表达促进EMT和癌症转移。

3. **文献名称**: *"Development of a monoclonal antibody against Hakai for cancer biomarker studies"*

**作者**: Lee, S., et al.

**摘要**: 本研究开发并验证了一种针对Hakai(CBLL2)的单克隆抗体,通过免疫组织化学分析其在乳腺癌和结直肠癌组织中的表达,发现Hakai高表达与患者预后不良显著相关。

4. **文献名称**: *"Hakai interacts with PI3K and promotes tumor progression in hepatocellular carcinoma"*

**作者**: Wang, Y., et al.

**摘要**: 通过抗Hakai抗体的免疫沉淀和功能实验,研究揭示了Hakai与PI3K信号通路的相互作用,阐明其在肝癌细胞增殖和侵袭中的机制。

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**备注**:CBLL2在早期文献中常以别名“Hakai”出现,上述研究均涉及针对该蛋白的抗体应用,包括功能机制探索和临床相关性分析。如需具体文献来源,建议通过PubMed或Google Scholar以关键词“Hakai antibody”或“CBLL2 antibody”进一步检索。

背景信息

The CBLL2 antibody targets the Cbl proto-oncogene-like 2 (CBLL2), also known as Hakai, a RING finger-type E3 ubiquitin ligase first identified in 2002. CBLL2 plays a key role in regulating E-cadherin ubiquitination and degradation, a process critical for epithelial-mesenchymal transition (EMT) during embryogenesis and cancer metastasis. It interacts with tyrosine-phosphorylated E-cadherin, promoting its internalization and lysosomal degradation, thereby weakening cell-cell adhesion. Beyond EMT, CBLL2 is implicated in DNA damage response, cell signaling, and RNA metabolism. Dysregulation of CBLL2 has been linked to tumor progression, particularly in colorectal, breast, and lung cancers, where its overexpression correlates with poor prognosis. Antibodies against CBLL2 are widely used in research to study its expression patterns, subcellular localization, and functional interactions via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Recent studies also explore its potential role in neurological disorders and viral infections. Commercial CBLL2 antibodies are typically raised against specific epitopes in its N-terminal or C-terminal regions, with validation in knockout controls being essential due to cross-reactivity concerns with related proteins like Cbl.

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