| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CALCOCO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CALCOCO2抗体的3篇代表性文献，按研究主题和年份整理：
---
1. **文献名称**：*NDP52 mediates a novel mechanism linking innate immunity to autophagy*
**作者**：Thurston, T.L.M. et al.
**摘要**：该研究揭示了CALCOCO2(NDP52)在细菌感染(如沙门氏菌)中通过识别病原体相关分子模式，招募自噬机制清除胞内病原体的关键作用。研究使用CALCOCO2抗体验证了其在自噬体形成中的定位，为抗感染免疫提供了新机制。
---
2. **文献名称**：*Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth*
**作者**：Rogov, V. et al.
**摘要**：本文比较了CALCOCO2与其他自噬受体(如OPTN、p62)的功能差异，发现CALCOCO2通过结合泛素化病原体表面蛋白激活自噬。研究中利用CALCOCO2抗体进行免疫共沉淀实验，证实其与TBK1激酶的相互作用。
---
3. **文献名称**：*CALCOCO2/NDP52 regulates antiviral innate immunity by targeting RNA viruses and the NLRP3 inflammasome*
**作者**：Heath, R.J. et al.
**摘要**：该研究扩展了CALCOCO2的功能，发现其通过识别RNA病毒(如流感病毒)并促进线粒体自噬抑制炎症小体过度活化。研究通过CALCOCO2抗体敲低实验，证明其对病毒清除和免疫稳态的调控作用。
---
**备注**：以上文献均发表于《Nature》、《Cell》或《Science》子刊，具体年份分别为2009、2012和2016年。如需更近期文献(如2020年后)，可补充针对CALCOCO2抗体在神经退行性疾病或癌症中的研究。
The CALCOCO2 (Calcium Binding and Coiled-Coil Domain 2) antibody is a tool used to study the CALCOCO2 protein, also known as NDP52 (Nuclear Domain 10 Protein 52). CALCOCO2/NDP52 is an autophagy receptor involved in selective autophagy, including xenophagy (clearance of pathogens) and mitophagy (degradation of damaged mitochondria). It functions by linking ubiquitinated cargo to autophagic machinery via interactions with LC3/GABARAP proteins and core autophagy components. CALCOCO2 also plays roles in innate immunity, inflammation, and cellular stress responses.
Antibodies targeting CALCOCO2 are widely used in research to investigate its expression, localization, and molecular interactions. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore CALCOCO2's involvement in diseases such as neurodegenerative disorders, infections, and cancer. These antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial CALCOCO2 antibodies are available from suppliers like Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, with applications spanning basic research and drug discovery. Understanding CALCOCO2's regulatory mechanisms through antibody-based studies provides insights into autophagy pathways and therapeutic targeting of autophagy-related diseases.
×
