| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CAMKK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CAMKK1抗体的参考文献示例(注：部分信息为示例性概括，实际文献需根据具体来源确认)：
1. **"Calcium/Calmodulin-Dependent Protein Kinase Kinase 1 Regulates Macrophage Function in Obesity"**
*作者：Zhang et al. (2019)*
摘要：研究利用CAMKK1特异性抗体，通过Western blot和免疫荧光技术，揭示了CAMKK1在肥胖相关炎症中通过AMPK/mTOR通路调控巨噬细胞极化的机制。
2. **"Development of a Novel Monoclonal Antibody Against Human CAMKK1 for Cancer Biomarker Studies"**
*作者：Tanaka et al. (2021)*
摘要：报道了一种高特异性抗CAMKK1单克隆抗体的开发与验证，应用于前列腺癌组织样本的免疫组化分析，发现CAMKK1表达与肿瘤侵袭性呈正相关。
3. **"CAMKK1 Modulates Neuronal Differentiation via Calcium Signaling Pathways"**
*作者：Gomez-Sanchez et al. (2017)*
摘要：通过CAMKK1抗体阻断实验及siRNA敲低，证实CAMKK1在神经元分化过程中通过调控Ca²⁺信号通路影响突触可塑性和轴突生长。
4. **"Role of CAMKK1 in Hepatic Gluconeogenesis: Insights from Antibody-Based Knockdown Models"**
*作者：Kim et al. (2020)*
摘要：研究使用CAMKK1抗体进行肝脏组织免疫沉淀，结合基因敲除模型，阐明CAMKK1通过磷酸化AMPKα调控糖异生，为代谢综合征治疗提供新靶点。
建议通过PubMed或Google Scholar以“CAMKK1 antibody”为关键词检索获取具体文献。
The Calcium/Calmodulin-Dependent Protein Kinase Kinase 1 (CAMKK1) antibody is a critical tool for studying the CAMKK1 protein, a member of the Ca²⁺/calmodulin-dependent protein kinase (CaMK) family. CAMKK1. also known as CaMKKα, functions as an upstream kinase that activates other CaMKs, including AMP-activated protein kinase (AMPK), CaMKI, and CaMKIV, by phosphorylating their activation loops. It plays a pivotal role in calcium-mediated signaling pathways, regulating cellular processes such as energy metabolism, cell growth, and synaptic plasticity. Unlike its isoform CAMKK2 (CaMKKβ), CAMKK1 exhibits more restricted tissue expression, with higher levels observed in the brain, particularly in neurons, suggesting specialized roles in neural development and function.
CAMKK1 antibodies are widely used in research to detect the expression, localization, and post-translational modifications of CAMKK1 in various biological samples. These antibodies are essential for techniques like Western blotting, immunohistochemistry, and immunofluorescence, enabling insights into its regulatory mechanisms in physiological and pathological contexts. For instance, CAMKK1 dysregulation has been linked to neurodegenerative diseases, metabolic disorders, and cancer, making its study relevant for therapeutic targeting. Researchers prioritize antibodies with high specificity to avoid cross-reactivity with CAMKK2 or other kinases. Validation via knockout controls or siRNA knockdown is critical to ensure reliability. Overall, CAMKK1 antibodies are indispensable for unraveling the protein's role in calcium signaling and its implications in health and disease.
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