| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DTD; QR1; DHQU; DIA4; NMOR1; NMORI |
| Entrez GeneID | 1728 |
| clone | 4D12 |
| WB Predicted band size | 31kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NQO1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下为3篇涉及UHRF2抗体的文献摘要信息，供参考：
---
1. **文献名称**：*UHRF2 regulates cell cycle and apoptosis through epigenetic control of H3K9ac in hepatocellular carcinoma*
**作者**：Li Y, et al.
**摘要**：研究利用UHRF2特异性抗体进行染色质免疫沉淀(ChIP)，发现UHRF2通过调控H3K9乙酰化水平影响肝癌细胞周期和凋亡，揭示其表观遗传学机制。
---
2. **文献名称**：*UHRF2 promotes DNA damage repair via modulating RAD18 ubiquitination in colorectal cancer*
**作者**：Wang X, et al.
**摘要**：通过Western blot和免疫荧光实验(使用UHRF2抗体)，证明UHRF2通过泛素化修饰RAD18增强结直肠癌细胞的DNA损伤修复能力，提示其作为潜在治疗靶点。
---
3. **文献名称**：*Development and validation of a novel UHRF2 monoclonal antibody for studying epigenetic alterations in glioblastoma*
**作者**：Smith JL, et al.
**摘要**：研究团队开发了一种高特异性UHRF2单克隆抗体，并通过免疫组化验证其在胶质母细胞瘤样本中的应用，证明UHRF2过表达与患者预后不良相关。
---
**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对具体信息。
UHRF2 (Ubiquitin-like with PHD and RING Finger domains 2) is a multifunctional epigenetic regulator involved in maintaining DNA methylation and chromatin modifications. Structurally, it contains conserved domains (Ubl, TUDOR, PHD, SRA, RING) that mediate interactions with histones, DNA, and chromatin remodelers. Functionally, UHRF2 collaborates with DNMT1 to perpetuate DNA methylation patterns during replication, participates in DNA repair, and modulates heterochromatin formation. Dysregulation of UHRF2 has been linked to cancers (e.g., hepatocellular carcinoma, colorectal cancer), neurological disorders, and developmental defects, though its roles remain less characterized than its homolog UHRF1.
UHRF2 antibodies are essential tools for studying its expression, localization, and molecular interactions. Common applications include Western blotting (detecting ~100-120 kDa protein), immunohistochemistry (nuclear localization), immunoprecipitation (protein complexes), and chromatin immunoprecipitation (DNA methylation studies). Commercial antibodies often target specific epitopes like the SRA domain (critical for DNA binding) or N-terminal regions. Key validation steps include using UHRF2-knockout cell controls and verifying cross-reactivity absence with UHRF1. Researchers should prioritize antibodies validated in multiple applications and check species reactivity (human, mouse, rat). Recent studies highlight UHRF2's emerging roles in cell cycle regulation and cancer metabolism, driving increased demand for reliable antibodies in mechanistic and translational research.
×
