| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human WDR59 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于WDR59抗体的3篇示例参考文献(内容基于模拟文献，可能非真实存在，仅供参考格式和方向)：
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**1. 文献名称**: *WDR59 regulates ciliogenesis through interaction with mTOR signaling components*
**作者**: Liu Y, et al.
**摘要**: 本研究利用WDR59特异性抗体，揭示了WDR59在纤毛形成中的调控作用。通过免疫共沉淀和免疫荧光技术，发现WDR59与mTOR复合物1(mTORC1)存在相互作用，并影响纤毛发生相关蛋白的定位。研究提示WDR59可能作为mTOR通路与细胞极性调控的桥梁分子。
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**2. 文献名称**: *Antibody-based profiling of WDR59 expression in human cancers*
**作者**: Chen H, et al.
**摘要**: 该研究开发了一种高特异性的WDR59单克隆抗体，用于分析其在多种癌症组织中的表达水平。结果显示，WDR59在结直肠癌和乳腺癌中显著高表达，且与患者预后不良相关。体外实验进一步证明，WDR59抗体可通过阻断其与CUL4-E3泛素连接酶的相互作用抑制肿瘤细胞增殖。
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**3. 文献名称**: *Structural characterization of WDR59 and its role in autophagy-lysosomal degradation*
**作者**: Martinez R, et al.
**摘要**: 通过结合冷冻电镜和WDR59抗体的免疫标记技术，本研究解析了WDR59蛋白的三维结构，并发现其WD40结构域对自噬体成熟至关重要。抗体阻断实验表明，WDR59缺失会导致自噬溶酶体形成障碍，提示其在细胞代谢稳态中的关键作用。
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(注：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。)
The WDR59 antibody is a research tool designed to detect and study the WD repeat-containing protein 59 (WDR59), a component of the GATOR2 complex in the mTORC1 signaling pathway. WDR59 contains conserved WD40 repeat motifs, which mediate protein-protein interactions, and plays a role in nutrient sensing by regulating mTORC1 activity. As part of GATOR2. WDR59 interacts with other subunits like WDR24 and MIOS to inhibit the upstream negative regulator GATOR1. thereby activating mTORC1 under amino acid-sufficient conditions. Dysregulation of this pathway is linked to cancer, metabolic disorders, and neurodegenerative diseases.
WDR59 antibodies are typically developed in host species (e.g., rabbits, mice) using immunogenic peptides or recombinant protein fragments. They enable applications such as Western blotting, immunoprecipitation, and immunofluorescence to analyze WDR59 expression, localization, and interactions in cell lines or tissues. Commercial antibodies often undergo validation for specificity via knockout controls or siRNA knockdown. However, variability in antibody performance across studies has been reported, emphasizing the need for rigorous validation. Research using WDR59 antibodies has expanded insights into its tissue-specific roles, post-translational modifications, and potential therapeutic targeting in diseases like cancer. Current challenges include elucidating its precise molecular mechanisms and resolving discrepancies in pathway models.
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