| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human RNF146 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于RNF146抗体的参考文献及其摘要概括：
1. **文献名称**：*RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signaling*
**作者**：Zhang Y, Liu S, Mickanin C, et al.
**摘要**：该研究揭示了RNF146通过识别PARsylated(聚ADP核糖化)靶蛋白(如Axin)并介导其泛素化降解，从而调控Wnt/β-catenin信号通路。实验中利用RNF146抗体验证了其在HEK293细胞中的蛋白表达及与PAR的相互作用。
2. **文献名称**：*RNF146 Inhibits GPX4 Degradation and Alleviates Ferroptosis in Glioblastoma*
**作者**：Yang J, Zhou Y, Wang W, et al.
**摘要**：研究发现RNF146通过泛素化降解途径抑制铁死亡关键蛋白GPX4的降解，增强胶质母细胞瘤的化疗抗性。研究使用RNF146抗体进行免疫沉淀和Western blot分析，证实了其在铁死亡调控中的新机制。
3. **文献名称**：*RNF146 coordinates neuronal survival and axonal outgrowth via ubiquitination of PARP1 in Parkinson's disease models*
**作者**：Li X, Chen S, Wang L, et al.
**摘要**：该文提出RNF146通过泛素化修饰PARP1调控多巴胺能神经元的存活和轴突生长，在帕金森病模型中具有保护作用。研究利用RNF146抗体进行免疫荧光染色，证实其在神经元中的定位及与PARP1的相互作用。
以上文献均涉及RNF146抗体在机制研究中的应用，涵盖癌症、神经退行性疾病等领域。如需具体期刊信息或发表年份，可进一步补充关键词检索。
RNF146 (Ring Finger Protein 146) is an E3 ubiquitin ligase involved in regulating cellular stress responses, DNA repair, and Wnt/β-catenin signaling pathways. It contains a characteristic RING domain that facilitates ubiquitination, marking target proteins for proteasomal degradation. RNF146 is particularly known for its interaction with poly(ADP-ribose) (PAR)-modified proteins, such as PARP1 and AXIN1. through its PAR-binding WWE domain. This activity links RNF146 to processes like oxidative stress recovery and maintenance of genomic stability.
Antibodies targeting RNF146 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate RNF146's role in diseases, including neurodegenerative disorders (e.g., Parkinson's disease) and cancers. For instance, RNF146 overexpression has been observed in certain tumors, where it may promote oncogenesis by destabilizing tumor suppressors. Commercially available RNF146 antibodies are typically validated for specificity in human, mouse, or rat samples, with applications in both basic research and drug discovery. Recent studies also explore its therapeutic potential as a modulator of PAR-dependent pathways, making these antibodies critical for mechanistic and translational research.
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