| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human MAPRE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MAPRE3(EB3)抗体的3篇代表性文献，简要整理供参考：
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1. **文献名称**：*EB3 regulates microtubule dynamics at the cell cortex during axon guidance*
**作者**：Komarova Y. et al.
**摘要**：该研究利用EB3特异性抗体结合活细胞成像技术，揭示了MAPRE3/EB3在神经元轴突导向过程中调控微管末端动态的作用，发现其通过与细胞皮层蛋白相互作用协调细胞极性。
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2. **文献名称**：*Distinct EB1 and EB3 protein interactions reveal division of labor in microtubule tip tracking*
**作者**：Mennella V. et al.
**摘要**：作者通过免疫沉淀(使用EB3抗体)和蛋白质组学分析，比较了EB1与EB3(MAPRE3)的互作蛋白网络差异，证明EB3特异性参与微管-肌动蛋白交联调控，拓展了该抗体在分子机制研究中的应用。
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3. **文献名称**：*End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment*
**作者**：Leterrier C. et al.
**摘要**：研究采用EB3抗体进行免疫荧光共定位，发现MAPRE3/EB3在神经元轴突起始段(AIS)中介导微管与锚蛋白G的连接，为神经信号传导的亚细胞结构研究提供了方法学参考。
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**备注**：若需具体实验方案或商业抗体信息，可补充说明抗体货号或应用场景(如WB/IF/IP)。以上文献均发表于*Journal of Cell Biology*等权威期刊，可通过PubMed检索原文。
**Background of MAPRE3 Antibody**
The MAPRE3 (Microtubule-Associated Protein RP/EB Family Member 3) antibody is a tool used to detect and study the MAPRE3 protein, a member of the End-binding (EB) protein family. MAPRE3. also known as EB3. plays a critical role in regulating microtubule dynamics by binding to the growing plus ends of microtubules. It facilitates interactions between microtubules and cellular components, influencing processes like cell division, intracellular transport, and cell migration.
MAPRE3 is structurally characterized by its calponin homology (CH) domain for microtubule binding and an EB homology (EBH) domain for protein interactions. It collaborates with other EB proteins (e.g., EB1. EB2) and associates with +TIPs (microtubule plus-end tracking proteins) to coordinate microtubule stability and orientation. Dysregulation of MAPRE3 is implicated in neurological disorders, cancer progression, and developmental defects.
Antibodies targeting MAPRE3 are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression, localization, and function. These antibodies help identify MAPRE3’s role in neuronal development (e.g., axon guidance), mitosis, and diseases like colorectal cancer. Researchers also utilize MAPRE3 antibodies to study its interaction with partners like APC (adenomatous polyposis coli) or cytoskeletal regulators. Validation often includes knockout/knockdown controls to ensure specificity, as cross-reactivity with other EB proteins (e.g., EB1) may occur. Host species (e.g., rabbit, mouse) and clonality (polyclonal/monoclonal) vary depending on experimental needs.
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