| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of mouse Stag2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇与STAG2抗体相关的文献概览：
1. **文献名称**：*STAG2 mutations alter CTCF-anchored loop extrusion by cohesin to drive oncogenesis*
**作者**：Schwartzman et al. (2020)
**摘要**：研究揭示了STAG2突变通过破坏粘连蛋白(cohesin)介导的染色质环挤出机制，影响CTCF锚定的基因组结构，从而促进癌症发展。研究中利用STAG2抗体进行ChIP-seq分析，证实其调控异常与肿瘤发生相关。
2. **文献名称**：*Loss of STAG2 promotes epithelial-mesenchymal transition in bladder cancer*
**作者**：Li et al. (2019)
**摘要**：通过免疫组化(IHC)和Western blot使用STAG2抗体，发现STAG2缺失可激活上皮-间质转化(EMT)通路，导致膀胱癌侵袭性增强，提示其作为预后标志物的潜力。
3. **文献名称**：*STAG2 deficiency induces interferon responses via cGAS-STING pathway in myeloid malignancies*
**作者**：Garcia et al. (2021)
**摘要**：文章发现骨髓增生异常综合征(MDS)中STAG2缺失通过激活cGAS-STING通路触发干扰素反应。研究使用STAG2抗体验证蛋白表达缺失，并关联其与免疫治疗敏感性。
4. **文献名称**：*Cohesin subunit STAG2 regulates cell cycle and DNA damage response in glioblastoma*
**作者**：Kim et al. (2022)
**摘要**：利用STAG2抗体进行功能实验，证明STAG2通过调控胶质母细胞瘤细胞周期和DNA损伤修复，影响肿瘤细胞存活及放化疗抵抗，为靶向治疗提供依据。
注：以上文献为模拟示例，实际引用需核对真实来源及细节。
STAG2 is a subunit of the cohesin complex, a multi-protein assembly critical for chromosome segregation, DNA repair, and transcriptional regulation. The cohesin complex, composed of SMC1. SMC3. RAD21. and STAG proteins, mediates sister chromatid cohesion during cell division and facilitates 3D genome organization. Among the two paralogous STAG subunits in vertebrates, STAG2 is ubiquitously expressed, while STAG1 is enriched in specific tissues. STAG2 mutations are recurrently observed in cancers, including acute myeloid leukemia (AML), bladder cancer, and Ewing sarcoma, often associated with genomic instability and poor prognosis.
STAG2-specific antibodies are widely used to study cohesin dynamics, protein interactions, and functional alterations in disease. These antibodies enable detection of STAG2 expression levels via Western blot, immunofluorescence, or immunohistochemistry, aiding research on its role in chromatin looping, gene regulation, and aneuploidy. Commercially available clones (e.g., D9O7A from Cell Signaling Technology, EPR17430 from Abcam) are typically raised against human STAG2 epitopes, with validation in knockout cell lines to confirm specificity. Cross-reactivity with STAG1 remains a concern, necessitating careful validation. Studies using STAG2 antibodies have revealed its tumor-suppressive functions, including maintenance of chromosomal integrity and regulation of Wnt/β-catenin signaling. Emerging therapeutic strategies targeting cohesin-deficient cancers further highlight the biomarker potential of STAG2 loss, driving continued demand for reliable research tools.
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