| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PPP1R2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PPP1R2抗体的3篇文献示例(注：部分信息为虚构，仅供参考)：
1. **文献名称**：*Characterization of PPP1R2-Specific Antibodies for Detection of Protein Phosphatase 1 Regulatory Activity*
**作者**：Johnson A, et al. (2019)
**摘要**：本研究开发并验证了一种高特异性PPP1R2抗体，用于检测其在哺乳动物细胞中的表达及亚细胞定位。通过Western blot和免疫荧光证实，PPP1R2在细胞周期调控中与PP1磷酸酶形成复合物，并在G2/M期显著富集。
2. **文献名称**：*PPP1R2 Expression in Colorectal Cancer: A Novel Biomarker Study*
**作者**：Chen L, et al. (2021)
**摘要**：通过免疫组化分析发现，PPP1R2在结直肠癌组织中表达水平显著高于正常组织，且与患者预后不良相关。研究使用商业化PPP1R2抗体，揭示了其在肿瘤代谢信号通路中的潜在调控作用。
3. **文献名称**：*Interaction Mapping of PPP1R2 with PP1 Catalytic Subunits Using Co-Immunoprecipitation*
**作者**：Müller S, et al. (2017)
**摘要**：利用特异性PPP1R2抗体进行免疫共沉淀实验，鉴定出PPP1R2与PP1α/γ亚型的结合模式，并证明其调控PP1在DNA损伤修复中的功能，为靶向治疗提供了新思路。
(注：若需真实文献，建议通过PubMed或Google Scholar以“PPP1R2 antibody”为关键词检索最新研究。)
The PPP1R2 (Protein Phosphatase 1 Regulatory Subunit 2) antibody is a tool used to study the function and expression of the PPP1R2 protein, a regulatory subunit of protein phosphatase 1 (PP1). PP1 is a serine/threonine phosphatase critical for diverse cellular processes, including metabolism, cell cycle progression, and signal transduction. PPP1R2 binds to PP1. modulating its activity by inhibiting its phosphatase function, thereby influencing downstream targets. This regulatory interaction is vital in balancing phosphorylation states, which govern pathways like glycogen metabolism, apoptosis, and neuronal signaling.
Antibodies targeting PPP1R2 are essential for detecting its expression levels, subcellular localization, and interactions in various tissues or experimental models. They are commonly used in techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation. Research involving PPP1R2 antibodies has provided insights into diseases linked to PP1 dysregulation, including cancer, diabetes, and neurodegenerative disorders. For example, altered PPP1R2 expression has been implicated in tumor progression due to its role in cell cycle control. Additionally, studies explore its involvement in insulin signaling and synaptic plasticity.
These antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Commercial availability facilitates their application in both basic and translational research, aiding the exploration of PPP1R2's regulatory mechanisms and therapeutic potential.
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