| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human LEPR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于LEPR抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *Structural basis for leptin receptor activation by the ultrapotential antibody antagonist*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过冷冻电镜解析了LEPR胞外结构域与拮抗性抗体的复合物结构，揭示了抗体结合LEPR后阻断瘦素信号的具体机制，为开发肥胖相关药物提供了结构基础。
2. **文献名称**: *LEPR-specific antibodies reverse leptin resistance in diet-induced obese mice*
**作者**: Zhao S, et al.
**摘要**: 团队开发了靶向LEPR特定表位的单克隆抗体，在肥胖小鼠模型中成功恢复了瘦素敏感性，证明抗体可通过调节受体二聚化状态改善代谢功能障碍。
3. **文献名称**: *Autoantibodies against leptin receptor in patients with metabolic syndrome*
**作者**: Tanaka M, et al.
**摘要**: 首次报道代谢综合征患者血清中存在抗LEPR自身抗体，这些抗体通过干扰JAK-STAT信号通路导致瘦素抵抗，提示自身免疫机制在肥胖病理中的作用。
4. **文献名称**: *Epitope mapping of leptin receptor using phage display antibodies*
**作者**: Guo L, et al.
**摘要**: 利用噬菌体展示技术筛选出高亲和力LEPR抗体，通过表位定位发现CRH2结构域的关键结合位点，为开发诊断用检测抗体提供了新策略。
Leptin receptor (LEPR) antibodies are tools used to study the leptin signaling pathway, which plays a critical role in regulating energy homeostasis, metabolism, and neuroendocrine function. LEPR, encoded by the *LEPR* gene, is a single-pass transmembrane receptor belonging to the class I cytokine receptor family. It binds leptin, an adipocyte-secreted hormone, to mediate downstream signaling via JAK-STAT, MAPK, and PI3K pathways. Dysregulation of leptin-LEPR interactions is linked to obesity, insulin resistance, and reproductive disorders.
LEPR exists in multiple isoforms due to alternative splicing, with the long isoform (LEPRb) being the primary signaling form in the hypothalamus. Antibodies targeting LEPR are designed to detect specific isoforms, phosphorylation states, or extracellular/ intracellular domains. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to investigate LEPR expression patterns in tissues, cellular localization, and signaling dynamics.
Research applications include studying metabolic diseases, leptin resistance mechanisms, and LEPR mutations associated with rare genetic disorders like congenital leptin receptor deficiency. Commercial LEPR antibodies vary in specificity and validation, requiring careful selection based on experimental needs. Challenges remain in distinguishing isoforms and minimizing cross-reactivity, underscoring the importance of antibody validation in leptin-related research.
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