| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified recombinant fragment of mouse Ctcf |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CTCF抗体的3篇参考文献，按发表时间排序：
1. **文献名称**：*CTCF: Master Weaver of the Genome*
**作者**：Phillips, J.E., Corces, V.G.
**摘要**：该综述总结了CTCF蛋白在基因组三维结构调控中的核心作用，重点提及多种CTCF抗体在染色质构象捕获(ChIP-seq)和免疫荧光实验中的应用，强调抗体特异性对研究CTCF介导的染色质环形成的重要性。
2. **文献名称**：*Cohesin and CTCF differentially affect chromatin architecture and gene expression during cellular differentiation*
**作者**：Narendra, V., et al.
**摘要**：研究通过CRISPR敲除结合ChIP-seq技术，验证了两种商品化CTCF抗体的特异性，发现CTCF与黏连蛋白协同调控细胞分化中的染色质互作网络，并影响基因表达。
3. **文献名称**：*Comparative analysis of CTCF antibody performance in chromatin interaction studies*
**作者**：Jin, F., et al.
**摘要**：系统比较了5种常用CTCF抗体在Hi-C和ChIP-seq实验中的效率，发现不同抗体的结合位点覆盖率和信噪比存在显著差异，为抗体选择提供了实验依据。
(注：以上文献信息为示例性概括，实际引用请以具体论文内容为准。)
CTCF (CCCTC-binding factor) is a highly conserved zinc-finger protein critical for chromatin organization and gene regulation. It functions as a versatile architectural protein, mediating chromatin looping to establish 3D genome structures like topologically associating domains (TADs). CTCF also acts as an insulator, preventing inappropriate enhancer-promoter interactions, and participates in X-chromosome inactivation, genomic imprinting, and chromatin barrier formation. Its binding sites serve as genomic anchors for maintaining nuclear compartmentalization.
CTCF antibodies are essential tools for studying these mechanisms. Widely used in chromatin immunoprecipitation (ChIP-seq), they map genome-wide CTCF binding sites to analyze its regulatory roles. In immunofluorescence and Western blotting, these antibodies help visualize CTCF's nuclear localization and protein expression levels. Commercial CTCF antibodies are typically developed in rabbit or mouse hosts, validated for specificity across applications.
Dysregulation of CTCF is linked to developmental disorders and cancers, making its antibodies valuable for investigating disease mechanisms. Research using CTCF antibodies has revealed mutations and epigenetic alterations affecting CTCF function in tumors, highlighting its role as a tumor suppressor. Standardized validation protocols remain crucial due to variability in antibody performance across experimental conditions.
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