| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ENAH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ENAH抗体的3篇模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**: *ENAH promotes invasive protrusion formation and metastatic dissemination in triple-negative breast cancer*
**作者**: Smith A, et al.
**摘要**: 研究通过免疫组化(使用ENAH特异性抗体)发现ENAH在三阴性乳腺癌中高表达，并证实其通过调控肌动蛋白重塑促进肿瘤细胞侵袭和转移。抑制ENAH可减少小鼠模型中的肺转移。
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2. **文献名称**: *ENAH overexpression as a prognostic biomarker in colorectal cancer*
**作者**: Tanaka K, et al.
**摘要**: 该研究利用ENAH抗体对200例结直肠癌组织进行染色，发现ENAH高表达与患者总生存期缩短显著相关，提示其可作为预后标志物及潜在治疗靶点。
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3. **文献名称**: *Mechanistic role of ENAH in collective cell migration*
**作者**: Lee J, et al.
**摘要**: 通过CRISPR敲除和ENAH抗体共聚焦成像，揭示了ENAH在集体细胞迁移中协调细胞间连接与肌动蛋白动力学的作用，为发育生物学和癌症研究提供新见解。
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**备注**: 以上为模拟示例，实际文献请通过PubMed、Web of Science或Google Scholar检索关键词“ENAH antibody”、“ENAH cancer”或结合具体研究领域进行筛选。
The ENAH (Enabled homolog) antibody is a crucial tool in studying the role of the ENAH protein, a member of the Enabled/VASP (vasodilator-stimulated phosphoprotein) family involved in actin cytoskeleton regulation. ENAH, encoded by the *ENAH* gene, promotes actin polymerization and filopodia formation by interacting with the Arp2/3 complex and other cytoskeletal regulators. It plays key roles in cell adhesion, motility, and morphogenesis, making it essential in developmental processes, cancer metastasis, and immune cell function.
ENAH overexpression is linked to tumor invasiveness in cancers like breast, colon, and pancreatic cancer, where it facilitates invadopodia formation and extracellular matrix degradation. Its splice variants (e.g., ENAH-V1/V2) exhibit context-dependent roles in tumor progression. The ENAH antibody is widely used in techniques like Western blot, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and post-translational modifications in research models.
Commercially available ENAH antibodies are critical for elucidating its interaction networks (e.g., Rho GTPases, Wnt signaling) and validating its potential as a therapeutic target. Recent studies also explore its involvement in neurological disorders and developmental defects. Validation via knockout controls or siRNA knockdown ensures antibody specificity, addressing challenges like isoform cross-reactivity. Overall, ENAH antibodies advance understanding of cytoskeletal dynamics in health and disease.
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