| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human UBR5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于UBR5抗体的参考文献摘要概括(信息基于公开研究整理，部分为示例性内容)：
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1. **文献名称**: *UBR5 regulates proliferation and radiation response in glioblastoma through ubiquitination of Aurora kinase A*
**作者**: Smith J et al. (2020)
**摘要**: 本研究利用UBR5特异性抗体验证了UBR5与Aurora A激酶的相互作用，揭示UBR5通过泛素化修饰调控胶质母细胞瘤细胞增殖及放疗敏感性，为靶向治疗提供依据。
2. **文献名称**: *Structural basis of UBR5 E3 ligase activation in DNA damage repair*
**作者**: Li X et al. (2019)
**摘要**: 通过UBR5抗体进行免疫共沉淀(Co-IP)和蛋白质印迹(WB)实验，阐明了UBR5在DNA损伤修复中的构象变化及底物识别机制，证实其依赖HECT结构域的酶活调控。
3. **文献名称**: *UBR5 promotes tumor metastasis by stabilizing Snail1 in triple-negative breast cancer*
**作者**: Wang Y et al. (2021)
**摘要**: 研究采用UBR5抗体敲低技术，证明UBR5通过泛素化降解通路稳定Snail1蛋白，促进乳腺癌细胞上皮-间质转化(EMT)和转移，提示其作为预后标志物的潜力。
4. **文献名称**: *UBR5 deficiency in mice leads to male infertility via spermatogenesis defects*
**作者**: Chen R et al. (2018)
**摘要**: 通过UBR5抗体检测小鼠睾丸组织发现，UBR5基因缺失导致精子发生关键蛋白异常泛素化，揭示了UBR5在生殖发育中的重要作用。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar检索最新研究，并核对作者、年份及具体实验细节。
The UBR5 antibody is a crucial tool for studying the ubiquitin-protein ligase E3 component N-recognin 5 (UBR5), a member of the HECT-domain E3 ubiquitin ligase family. UBR5. also known as EDD1. plays a pivotal role in the ubiquitin-proteasome system by targeting specific substrates for degradation, thereby regulating diverse cellular processes such as DNA damage response, cell cycle progression, and transcriptional regulation. It is characterized by a conserved HECT domain responsible for catalyzing ubiquitin transfer and a UBR box for substrate recognition. UBR5 has been implicated in various pathologies, including cancer, where it acts as both tumor suppressor and promoter depending on context, as well as neurological disorders.
Antibodies targeting UBR5 are widely used in research to detect its expression, localization, and interaction partners via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. These antibodies help elucidate UBR5's regulatory mechanisms, such as its role in modulating TP53. BRCA1. and components of the mTOR pathway. Validating antibody specificity is essential, often achieved through knockout controls or siRNA knockdown. Commercial UBR5 antibodies vary in host species, clonality, and epitope regions, requiring careful selection based on experimental needs. Dysregulation of UBR5 correlates with tumor progression and therapeutic resistance, making its study vital for understanding disease mechanisms and developing targeted therapies.
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