Mouse Monoclonal AMBP Antibody

+ +

•  

   
     Western blot analysis using AMBP mouse mAb against  Hep G2 lysate    

•  

   
     Lane 1: Hep G2 lysate;  Lane 2: AMBP immunoprecipitated from Hep G2 lysate by P15346;  Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P15346 can immunoprecipitate AMBP;    

•  

   
     IHC-P analysis of liver tissue by AMBP antibody (P15346) IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue;Result: Hepatocytes are positively stained at the cytoplasm.    

The Alpha-1-Microglobulin/Bikunin Precursor (AMBP) protein is a liver-synthesized glycoprotein that undergoes proteolytic cleavage to generate two functional subunits: alpha-1-microglobulin (A1M) and bikunin. A1M exhibits antioxidant and immunomodulatory properties, binding heme and reactive oxygen species, while bikunin, a serine protease inhibitor, regulates inflammation by inhibiting enzymes like trypsin and plasmin. Both subunits are involved in extracellular matrix maintenance and stress response pathways.

AMBP antibodies are essential tools for detecting and quantifying these subunits in research and diagnostics. They enable the study of AMBP's roles in diseases such as chronic kidney disease, sepsis, and cancer, where altered expression or dysfunction is observed. For example, urinary A1M and bikunin levels are biomarkers for kidney injury, while bikunin's anti-metastatic effects are explored in oncology. Antibodies specific to AMBP or its cleavage products are used in techniques like ELISA, Western blot, and immunohistochemistry to assess protein localization, expression dynamics, and post-translational modifications. Their development emphasizes specificity to distinguish between isoforms or modified forms, ensuring accurate biological insights. Clinically, AMBP antibodies aid in diagnosing pathological states or monitoring therapeutic interventions targeting inflammation or oxidative stress pathways.