| WB | 咨询技术 | Mouse, Rat |
| IF | 咨询技术 | Mouse, Rat |
| IHC | 1/100-1/200 | Mouse, Rat |
| ICC | 技术咨询 | Mouse, Rat |
| FCM | 咨询技术 | Mouse, Rat |
| Elisa | 咨询技术 | Mouse, Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, Rat |
| Immunogen | Purified recombinant fragment of mouse Pou3f1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与Pou3f1抗体相关的参考文献示例(注：文献标题和作者为虚构示例，建议通过学术数据库核实真实文献)：
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1. **文献名称**: "Pou3f1 regulates neural progenitor differentiation through histone modification"
**作者**: Li et al., 2020
**摘要**: 本研究利用Pou3f1特异性抗体进行染色质免疫沉淀(ChIP-seq)和免疫荧光染色，发现Pou3f1通过结合特定基因组区域调控神经前体细胞的分化，并揭示其在小鼠大脑发育中的关键作用。
2. **文献名称**: "Antibody validation for Pou3f1 in mammalian embryonic tissues"
**作者**: Garcia-Rodriguez & Smith, 2018
**摘要**: 文章系统验证了Pou3f1抗体在哺乳动物胚胎组织(如小鼠和人类)中的特异性，通过Western blot和免疫组化证明该抗体可特异性识别Pou3f1蛋白，且适用于多种实验条件。
3. **文献名称**: "Pou3f1 expression dynamics in peripheral nerve regeneration"
**作者**: Wang et al., 2016
**摘要**: 通过Pou3f1抗体进行免疫组织化学分析，研究发现Pou3f1在周围神经损伤后显著上调，提示其可能参与轴突再生的调控机制。
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**建议**：以上为示例，真实文献可通过PubMed、Google Scholar等平台以关键词“Pou3f1 antibody”、“Pou3f1 immunohistochemistry”或“Pou3f1 function”检索，并筛选涉及抗体应用的实验研究。
Pou3f1. also known as Oct-6 or SCIP (suppressed cAMP-inducible POU), is a transcription factor belonging to the POU-domain protein family. It plays critical roles in nervous system development, particularly in the differentiation of oligodendrocytes and Schwann cells, which are essential for myelination in the central and peripheral nervous systems, respectively. Pou3f1 is expressed during specific developmental stages, regulating genes involved in cell cycle exit, maturation, and myelin formation. Dysregulation of Pou3f1 has been linked to neurodevelopmental disorders and demyelinating diseases.
Antibodies targeting Pou3f1 are widely used in neuroscience research to detect its expression patterns, localization, and interactions. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study Pou3f1's role in myelination, neural crest cell migration, and neural stem cell differentiation. Specificity and validation (e.g., knockout controls) are crucial, as cross-reactivity with other POU-family members (e.g., Oct-1 or Brn2) may occur. Researchers also utilize Pou3f1 antibodies to explore its involvement in disease models, such as Charcot-Marie-Tooth neuropathy or multiple sclerosis, providing insights into potential therapeutic targets. Commercial antibodies are typically raised against conserved epitopes within the N-terminal or POU-specific domains of the protein.
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