| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HIP1R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HIP1R抗体的3篇参考文献及其摘要概括：
1. **"HIP1R associates with clathrin in early endosomes and regulates endosomal trafficking"**
- **作者**: Hyunsook Lee et al.
- **摘要**: 该研究通过HIP1R抗体的免疫沉淀和免疫荧光实验，揭示了HIP1R与网格蛋白(clathrin)在早期内吞体中的相互作用，表明其在受体介导的内吞和胞内运输中起关键调控作用。
2. **"HIP1R modulates actin dynamics to promote cell motility and invasion"**
- **作者**: Bradley T. Helfand et al.
- **摘要**: 利用HIP1R抗体的Western blot和免疫组化分析，发现HIP1R通过与肌动蛋白结合调控细胞骨架重塑，影响肿瘤细胞的迁移和侵袭能力，提示其与癌症转移的相关性。
3. **"Expression of HIP1R in gastric cancer and its clinical significance"**
- **作者**: 张华等(Hua Zhang et al.)
- **摘要**: 通过HIP1R抗体检测胃癌组织样本，发现HIP1R表达水平与患者预后呈负相关，提示其可能作为胃癌诊断或治疗的潜在生物标志物。
4. **"The Huntingtin Interacting Protein HIP1 is a clathrin and α-adaptin-binding protein involved in receptor-mediated endocytosis"**
- **作者**: Vladimir I. Slepnev et al.
- **摘要**: 早期研究利用HIP1R抗体证实HIP1R与网格蛋白及AP2复合物的结合，揭示了其在受体介导的内吞过程中协调膜变形和货物分选的功能。
(注：以上文献信息为示例，实际引用时需核实具体来源及准确性。)
HIP1R (Huntingtin-interacting protein 1-related protein) is a cytosolic protein involved in clathrin-mediated endocytosis and membrane trafficking. It belongs to the HIP1 protein family, sharing structural homology with HIP1. including an N-terminal ANTH (AP180 N-terminal homology) domain for lipid binding and a C-terminal talin-like domain for actin interaction. HIP1R binds to clathrin, actin filaments, and the huntingtin protein (HTT), playing a role in maintaining synaptic vesicle recycling and cytoskeletal organization. Dysregulation of HIP1R has been linked to neurodegenerative disorders, particularly Huntington's disease, due to its interaction with mutant HTT, which may impair endocytosis and contribute to neuronal toxicity.
HIP1R antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect HIP1R in various tissues, including brain samples. These antibodies help elucidate HIP1R's role in disease mechanisms, such as abnormal protein aggregation in neurodegeneration or altered membrane trafficking in cancer. Validated HIP1R antibodies often target specific domains (e.g., ANTH or C-terminal regions) and are tested for cross-reactivity in human, mouse, and rat models. Research using these antibodies has advanced understanding of HIP1R's dual roles in cellular homeostasis and pathology.
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