| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NUB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NUB1抗体的3篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**: *NUB1. a NEDD8-interacting protein, is induced by interferon and facilitates proteasome activity*
**作者**: Kito K, et al.
**摘要**: 该研究首次报道了NUB1(NEDD8 Ultimate Buster 1)的发现及其在干扰素信号通路中的作用。研究通过制备NUB1特异性抗体，证实NUB1通过与NEDD8结合促进泛素-蛋白酶体系统的降解功能，并揭示了其在抗病毒反应中的潜在机制。
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2. **文献名称**: *Regulation of NUB1 stability and function through its interaction with the ubiquitin-like protein FAT10*
**作者**: Tanaka T, et al.
**摘要**: 本文利用NUB1抗体进行免疫共沉淀和Western blot分析，发现NUB1与泛素样蛋白FAT10相互作用，并参与调控蛋白质的稳定性与降解。研究还表明，NUB1的亚细胞定位依赖其C端结构域，抗体实验进一步支持其在蛋白酶体调控中的关键作用。
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3. **文献名称**: *Development and application of a monoclonal antibody specific for human NUB1*
**作者**: Mori-Konya C, et al.
**摘要**: 该研究开发了一种针对人源NUB1的单克隆抗体，验证了其在免疫组化、免疫荧光及流式细胞术中的高特异性和灵敏度。通过该抗体，研究揭示了NUB1在多种癌细胞系中的差异表达模式，并探讨其与肿瘤发生发展的相关性。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science等数据库核对具体信息，并优先选择近5年内的研究以反映最新进展。
NUB1 (Negative Regulator of Ubiquitin-like Proteins 1) is a cytoplasmic protein implicated in the ubiquitin-proteasome system (UPS), a critical pathway for protein degradation and cellular regulation. It functions as a scaffold protein, facilitating the recruitment of ubiquitin-like proteins (UBLs), such as NEDD8 and FAT10. to the proteasome for degradation. NUB1 achieves this by binding to these UBLs through its ubiquitin-associated (UBA) domain and interacting with proteasome components via its ubiquitin-like (UBL) domain. Its role in protein turnover and stress response has linked NUB1 to various cellular processes, including cell cycle regulation, apoptosis, and neurodegeneration.
Antibodies targeting NUB1 are essential tools for studying its expression, localization, and functional mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate NUB1’s involvement in diseases, particularly neurodegenerative disorders such as Parkinson’s disease. Studies suggest that NUB1 may modulate the clearance of misfolded proteins, including α-synuclein aggregates, by enhancing proteasomal activity. Dysregulation of NUB1 has been observed in pathological conditions, highlighting its potential as a therapeutic target or biomarker. Researchers also utilize NUB1 antibodies to explore its interactions with other UPS components and its regulatory effects on protein quality control. These antibodies enable precise detection of NUB1 isoforms and post-translational modifications, aiding in unraveling its complex roles in cellular homeostasis and disease.
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