| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human UBE2Z |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于UBE2Z抗体的3篇参考文献，涵盖其功能研究及在疾病中的应用：
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1. **"UBE2Z functions as a ubiquitin-conjugating enzyme in the ubiquitination of specific proteins"**
*Author: Smith J, et al.*
**摘要**：本研究验证了UBE2Z作为泛素结合酶(E2)的活性，并开发了特异性抗体用于检测其在细胞内的蛋白表达。通过免疫沉淀和Western blot分析，发现UBE2Z与特定E3连接酶相互作用，促进底物泛素化，为泛素化通路机制提供新见解。
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2. **"UBE2Z overexpression correlates with poor prognosis in hepatocellular carcinoma"**
*Author: Li X, et al.*
**摘要**：研究利用UBE2Z抗体对肝癌组织进行免疫组化分析，发现UBE2Z在肿瘤中显著高表达，并与患者生存率负相关。功能实验表明，UBE2Z通过调控NF-κB通路促进肿瘤增殖，提示其作为肝癌治疗靶点的潜力。
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3. **"Development of a monoclonal antibody against human UBE2Z for diagnostic applications"**
*Author: Tanaka K, et al.*
**摘要**：本文报道了一种高特异性和灵敏度的抗人UBE2Z单克隆抗体的开发。通过ELISA和免疫荧光验证，该抗体能有效识别天然和变性UBE2Z蛋白，适用于临床样本检测，为UBE2Z相关疾病的诊断提供工具。
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*注：上述文献信息为模拟示例，实际引用需以真实文献为准。建议通过PubMed或Google Scholar以关键词“UBE2Z antibody”或“UBE2Z function”检索最新研究。*
The UBE2Z antibody is a research tool designed to detect and study UBE2Z (Ubiquitin-Conjugating Enzyme E2 Z), a key enzyme in the ubiquitin-proteasome system. UBE2Z, also known as HSPC150 or E2-230K, functions as a ubiquitin-conjugating enzyme (E2) that works with E3 ligases to transfer ubiquitin molecules to substrate proteins, marking them for proteasomal degradation or regulating their activity. This post-translational modification, ubiquitination, plays critical roles in protein quality control, DNA repair, cell cycle regulation, and signal transduction.
UBE2Z is particularly notable for its involvement in forming atypical ubiquitin chains linked through lysine 29 (K29) or lysine 48 (K48), which influence substrate specificity and downstream processes. Dysregulation of UBE2Z has been implicated in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, where altered protein degradation pathways contribute to pathogenesis.
The UBE2Z antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate UBE2Z expression levels, localization, and interactions in cellular or tissue samples. Researchers employ it to explore UBE2Z's functional roles in disease models, therapeutic responses, and molecular mechanisms. Validated antibodies are essential for ensuring specificity, as cross-reactivity with other E2 enzymes could lead to misinterpretation. Commercial UBE2Z antibodies are typically raised against specific epitopes, with validation data provided for reliability across experimental applications.
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