| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human WWP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于WWP1抗体的虚构参考文献示例(仅供格式参考，实际文献请通过学术数据库检索)：
1. **文献名称**：*WWP1-mediated ubiquitination of the RNA polymerase II large subunit in mouse embryonic pluripotent stem cells*
**作者**：Chen et al., 2017
**摘要**：本研究利用WWP1特异性抗体进行免疫共沉淀(Co-IP)，揭示了WWP1通过泛素化修饰RNA聚合酶II大亚基调控胚胎干细胞多能性维持的分子机制。
2. **文献名称**：*The E3 ligase WWP1 regulates canonical Wnt signaling through the degradation of Dishevelled proteins*
**作者**：Li et al., 2019
**摘要**：通过Western blot和免疫荧光实验(使用兔源WWP1多克隆抗体)，作者发现WWP1通过靶向Dishevelled蛋白的泛素化降解，负向调控Wnt/β-catenin信号通路。
3. **文献名称**：*Development and validation of a monoclonal antibody for specific detection of WWP1 in human cancer tissues*
**作者**：Wang et al., 2020
**摘要**：报道了一种新型小鼠抗人WWP1单克隆抗体的开发，通过免疫组化验证其在乳腺癌和前列腺癌组织中的高特异性表达，并证实WWP1表达水平与患者预后相关。
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**提示**：实际检索时可尝试以下方法：
- 在PubMed或Google Scholar中搜索 **"WWP1 antibody" + "validation"/"application"**;
- 筛选侧重于抗体开发、蛋白互作或信号通路机制的研究;
- 关注期刊如*Cell Research*、*Nature Communications*或*Journal of Biological Chemistry*。
The WWP1 antibody is a crucial tool for studying the WW domain-containing E3 ubiquitin protein ligase 1 (WWP1), a member of the NEDD4 family of HECT-type E3 ubiquitin ligases. WWP1 plays a pivotal role in regulating protein ubiquitination, a post-translational modification essential for controlling protein degradation, localization, and activity. Structurally, WWP1 contains an N-terminal C2 domain (mediating membrane binding), multiple WW domains (facilitating substrate recognition), and a C-terminal HECT domain (catalyzing ubiquitin transfer). It targets substrates like PTEN, p53. and TGFβ, influencing pathways linked to cancer, neurodegeneration, and metabolic disorders. Dysregulation of WWP1 is implicated in tumor progression, where it often acts as an oncogene by degrading tumor suppressors or activating oncoproteins.
WWP1 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to detect WWP1 expression levels, subcellular localization, and interactions in cells or tissues. These antibodies are vital for exploring WWP1's roles in disease mechanisms, particularly in cancer research, where its overexpression correlates with poor prognosis. Researchers must validate antibody specificity due to potential cross-reactivity with homologous family members (e.g., WWP2) and confirm results using knockout controls. Commercial WWP1 antibodies are available in monoclonal and polyclonal formats, often targeting epitopes within the HECT or WW domains. Understanding WWP1's regulatory functions through such antibodies may guide therapeutic strategies targeting ubiquitination pathways.
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