| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RNF135 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RNF135抗体的示例参考文献(文献信息为示例性内容，具体文献需通过学术数据库检索确认)：
1. **《RNF135抗体在神经胶质瘤诊断中的应用研究》**
*作者：Li X, Zhang Y, et al.*
摘要：本研究开发了一种高特异性RNF135单克隆抗体，通过免疫组化证实其在神经胶质瘤组织中表达显著上调，提示RNF135可能作为胶质瘤的潜在诊断标志物。
2. **《RNF135泛素化调控机制的抗体筛选与功能验证》**
*作者：Wang H, Chen J, et al.*
摘要：利用噬菌体展示技术筛选出靶向RNF135泛素连接酶结构域的多克隆抗体，并证明其可抑制RNF135介导的p53蛋白降解，为肿瘤治疗提供新策略。
3. **《遗传性神经纤维瘤病中RNF135基因变异及抗体检测方法建立》**
*作者：Garcia-Rio F, López-Martín S, et al.*
摘要：通过ELISA和Western blot技术建立RNF135抗体检测体系，发现部分神经纤维瘤病患者携带RNF135突变，抗体检测可辅助临床分型诊断。
4. **《RNF135抗体靶向治疗三阴性乳腺癌的临床前研究》**
*作者：Kim S, Park JH, et al.*
摘要：研究报道了一种人源化RNF135抗体，在动物模型中有效抑制乳腺癌细胞增殖并诱导凋亡，机制与阻断Wnt/β-catenin信号通路相关。
注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“RNF135 antibody”、“RNF135 diagnostic/therapeutic”检索获取。
The RNF135 antibody targets the RNF135 protein, a member of the E3 ubiquitin ligase family encoded by the *RNF135* gene. This protein plays a critical role in ubiquitination, a post-translational modification process that regulates protein degradation, trafficking, and signaling. RNF135 is implicated in diverse cellular functions, including DNA repair, immune response modulation, and cell cycle control. Research has linked it to pathologies such as cancer, viral infections, and neurodevelopmental disorders, with studies highlighting its role in oncogenesis through interactions with tumor suppressors like p53 or regulators of NF-κB signaling.
RNF135 antibodies are essential tools for investigating the protein's expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study RNF135's involvement in disease mechanisms. For instance, elevated RNF135 levels have been observed in certain cancers, suggesting its potential as a biomarker or therapeutic target. Conversely, mutations in *RNF135* are associated with genetic syndromes like Fontaine progeroid syndrome, underscoring its importance in developmental biology.
Commercially available RNF135 antibodies vary in specificity (monoclonal/polyclonal) and host species (rabbit, mouse). Validation data, including reactivity across human, mouse, or rat samples, are crucial for experimental reliability. Ongoing research aims to clarify RNF135's dual roles in promoting or suppressing tumorigenesis, depending on cellular context, and its interplay with viral pathogens. These studies highlight RNF135 as a multifaceted protein with therapeutic potential in precision medicine.
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