Mouse Monoclonal NEDD4 Antibody

The NEDD4 (Neuronal Precitor Cell-Expressed Developmentally Downregulated 4) antibody is a critical tool for studying the NEDD4 family of E3 ubiquitin ligases, which play pivotal roles in protein ubiquitination and degradation. NEDD4. a member of the HECT-domain E3 ligase family, regulates cellular processes such as membrane receptor trafficking, apoptosis, and cell proliferation by tagging target proteins (e.g., PTEN, EGFR, and Notch) with ubiquitin for proteasomal or lysosomal degradation. Its structure includes a C2 domain (mediating membrane binding), multiple WW domains (substrate recognition), and a catalytic HECT domain. Dysregulation of NEDD4 is implicated in cancers, neurological disorders (e.g., Alzheimer’s and Parkinson’s), and hypertension. Researchers employ NEDD4 antibodies in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to investigate its expression, interaction partners, and subcellular localization. These antibodies help elucidate NEDD4’s role in disease mechanisms and validate its potential as a therapeutic target. Specificity validation (via knockout controls) and cross-reactivity checks are essential due to homology among NEDD4 family members (NEDD4-1. NEDD4-2. etc.).