关键信息
-
基因名
Neuregulin-4/NRG4
-
简介
Neuregulin-4 (NRG4) Protein, a low-affinity ligand for the ERBB4 tyrosine kinase receptor, serves as a signaling molecule that recruits ERBB1 and ERBB2 coreceptors. This recruitment leads to ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. NRG4 specifically interacts with the ERBB4 receptor, mediating cellular responses through its activation, without binding to ERBB1, ERBB2, and ERBB3 receptors. Neuregulin-4/NRG4 Protein, Human is the recombinant human-derived Neuregulin-4/NRG4 protein, expressed by E. coli, with tag free.
- 应用
-
生物活性
Measured in a cell proliferation assay using Raji human Burkitt's lymphoma cells. The ED50 for this effect is 0.0144 μg/mL, corresponding to a specific activity is 6.944×104 units/mg. Measured in a cell proliferation assay using Raji human Burkitt's lymphoma cells. The ED50 this effect is 0.0144 μg/mL, corresponding to a specific activity is 6.944×10^4 units/mg.
-
别名
Pro-neuregulin-4, membrane-bound isoform; Pro-NRG4; NRG-4
-
种属
Human
-
表达系统
E. coli
-
标签
Tag Free
-
纯度
Greater than 95% as determined by reducing SDS-PAGE.
-
蛋白编号
Q8WWG1
-
表达区间
Q8WWG1 (N-G&P, P2-F62)
-
蛋白长度
Partial
-
分子量
6.7-14 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Neuregulin-4 (NRG4) is a member of the neuregulin family, which plays a vital role in various physiological processes, including neuronal development, cardiac function, and immune regulation. Recent studies have highlighted NRG4's potential therapeutic applications due to its involvement in metabolic pathways and its protective effects against obesity, insulin resistance, and inflammation. Research indicates that NRG4 may enhance browning of white adipose tissue, thereby promoting energy expenditure and improving metabolic health. Its anti-inflammatory properties have also garnered interest in the context of various diseases, such as diabetes and cardiovascular disorders. The recombinant protein form of NRG4 enables researchers to investigate its biological functions and therapeutic effects in a controlled manner, facilitating studies on receptor interactions and downstream signaling pathways. Furthermore, recombinant NRG4 can be utilized in cell-based assays and animal models to elucidate its mechanisms of action, assess its therapeutic efficacy, and explore its potential as a biomarker for metabolic disorders. Overall, the investigation of NRG4 and its recombinant protein represents a promising avenue for advancing our understanding of metabolic regulation and developing new interventions for metabolic diseases.
