| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KRT4抗体的3-4篇参考文献的示例(注：以下内容为虚构示例，仅用于格式参考)：
1. **文献名称**: "KRT4 Expression in Esophageal Squamous Cell Carcinoma: A Prognostic Biomarker Study"
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化(IHC)和Western blot分析KRT4在食管鳞状细胞癌中的表达，发现KRT4抗体标记的角蛋白表达水平与肿瘤分化程度及患者预后显著相关，低表达提示更差的生存率。
2. **文献名称**: "Immunohistochemical Detection of KRT4 in Hereditary Mucosal Disorders"
**作者**: Zhang L, et al.
**摘要**: 文章利用KRT4抗体对遗传性黏膜疾病(如白色海绵状痣)患者的组织样本进行分析，证实KRT4基因突变导致角蛋白结构异常，抗体染色可用于辅助诊断此类罕见疾病。
3. **文献名称**: "KRT4 as a Marker of Epithelial Differentiation in Head and Neck Cancers"
**作者**: Brown C, et al.
**摘要**: 通过对比头颈部鳞状细胞癌中多种角蛋白的表达模式，发现KRT4抗体特异性识别高分化肿瘤区域，提示其在评估肿瘤分化状态中的潜在临床应用价值。
4. **文献名称**: "Comparative Study of KRT4 and KRT13 in Urothelial Carcinoma"
**作者**: Lee J, et al.
**摘要**: 研究比较了膀胱尿路上皮癌中KRT4和KRT13的表达差异，发现KRT4抗体在低级别肿瘤中呈现高表达，而高级别肿瘤中表达缺失，表明其可能作为分级辅助指标。
(注：实际文献需通过PubMed、Google Scholar等平台检索关键词“KRT4 antibody”或“Keratin 4 immunohistochemistry”获取。)
The KRT4 antibody targets keratin 4. a member of the type II intermediate filament keratin family encoded by the *KRT4* gene. Keratins are structural proteins critical for epithelial integrity, differentiation, and mechanical resilience. KRT4 is predominantly expressed in non-cornified stratified squamous epithelia, such as those in the oral mucosa, esophagus, and salivary glands, often pairing with KRT13 (type I) to form heterodimers. Its expression is closely linked to terminal differentiation, making it a marker for non-keratinizing epithelial cells.
In research and diagnostics, the KRT4 antibody is widely used to study epithelial biology, tissue-specific differentiation, and pathological conditions. It helps identify cellular origin in carcinomas (e.g., distinguishing squamous cell carcinomas from adenocarcinomas) and diagnose genetic disorders like white sponge nevus, caused by *KRT4* mutations. Commercial KRT4 antibodies are typically monoclonal or polyclonal, validated via immunohistochemistry, immunofluorescence, or Western blot. Specificity is confirmed using knockout controls or tissues with known expression patterns.
Recent studies also explore KRT4's role in tumor progression, cell adhesion, and its potential as a biomarker in cancers. Its dynamic expression in premalignant lesions underscores its diagnostic relevance. Overall, the KRT4 antibody serves as a vital tool in both basic epithelial biology and clinical pathology.
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