Mouse Monoclonal NCF2 Antibody

The NCF2 (Neutrophil Cytosolic Factor 2) antibody is a tool used to study the p67phox protein, encoded by the *NCF2* gene, which is a critical component of the NADPH oxidase complex. This enzyme complex, primarily active in phagocytes, generates reactive oxygen species (ROS) crucial for microbial killing and inflammatory regulation. NCF2/p67phox binds to other subunits (e.g., p22phox, p47phox) to activate NADPH oxidase, facilitating electron transfer for superoxide production. Mutations in *NCF2* disrupt ROS generation, leading to chronic granulomatous disease (CGD), a rare immunodeficiency characterized by recurrent infections and granuloma formation. NCF2 antibodies are employed in research to detect protein expression, localization, and interactions, aiding in understanding NADPH oxidase dysfunction in CGD and other conditions like autoimmune disorders (e.g., lupus) where aberrant ROS signaling contributes to pathogenesis. They also serve diagnostic purposes, identifying NCF2 deficiencies in clinical settings. Recent studies explore NCF2’s role beyond immunity, including its impact on cellular signaling and cancer progression. Commercial NCF2 antibodies are typically validated for techniques like Western blotting, immunofluorescence, or ELISA, with specificity varying by epitope and host species. Ongoing research continues to refine their applications in both basic and translational contexts.