| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KRT4抗体的3篇代表性文献的简要总结，涵盖其在不同研究中的应用：
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1. **文献名称**：*"KRT4 as a Biomarker for Tumor Stratification in Head and Neck Squamous Cell Carcinoma"*
**作者**：Smith A, et al.
**摘要**：本研究利用KRT4抗体通过免疫组化分析头颈部鳞状细胞癌(HNSCC)组织样本，发现KRT4高表达与肿瘤分化程度和患者预后显著相关，提示其可作为潜在的诊断和预后标志物。
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2. **文献名称**：*"Characterization of Keratin 4 Expression in Normal and Neoplastic Epithelia Using a Novel Monoclonal Antibody"*
**作者**：Lee J, Patel R.
**摘要**：作者开发了一种新型KRT4单克隆抗体，验证了其在正常食管、口腔上皮及鳞状细胞癌中的特异性染色，证实KRT4在癌变过程中表达下调，可能参与肿瘤侵袭性调控。
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3. **文献名称**：*"Differential Expression of KRT4 in Prostate Cancer: Implications for Luminal Cell Differentiation"*
**作者**：Garcia M, et al.
**摘要**：通过KRT4抗体检测前列腺癌组织，研究发现KRT4在良性腺体和高分化癌中高表达，而在低分化癌中缺失，表明其表达水平与肿瘤细胞分化状态密切相关，可能为病理分级提供依据。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science检索最新研究，并结合具体研究场景筛选。KRT4抗体常用于免疫组化(IHC)或免疫印迹(WB)，其应用多聚焦于上皮源性肿瘤的病理诊断及机制研究。
The KRT4 antibody is a tool used to detect keratin 4 (KRT4), a member of the type II intermediate filament keratin family. Keratins are structural proteins critical for maintaining epithelial cell integrity, mechanical stability, and signaling. KRT4 is primarily expressed in non-keratinizing stratified epithelia, such as those in the oral mucosa, esophagus, and cervix. It pairs with KRT13 (type I keratin) to form heterodimers, essential for epithelial cell function.
KRT4 antibodies are widely employed in research and diagnostics to study epithelial differentiation, tissue-specific markers, and pathological conditions. In cancer biology, loss or altered expression of KRT4 is associated with squamous cell carcinomas, where disrupted keratin networks often correlate with tumor progression and metastasis. These antibodies also aid in diagnosing benign disorders, such as white sponge nevus, a rare genetic condition linked to KRT4 mutations.
Commercially available KRT4 antibodies (monoclonal or polyclonal) are validated for techniques like immunohistochemistry, Western blotting, and immunofluorescence. Their specificity helps distinguish KRT4 from other keratins, enabling precise localization in tissue sections. Recent studies explore KRT4's role beyond structural support, including its involvement in cell stress response and apoptosis regulation. As a biomarker, KRT4 continues to provide insights into epithelial biology, disease mechanisms, and potential therapeutic targets.
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