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Mouse Monoclonal EED Antibody

  • 中文名: EED抗体
  • 别    名: nan
货号: IPDX15043
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/500-1/1000 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human EED
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是3篇与EED抗体相关的文献摘要概述,供参考:

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1. **文献名称**:*EZH2 and EED subunit-specific inhibitors regulate chromatin and gene expression in PRC2-dependent cancers*

**作者**:Qi W. et al.

**摘要**:该研究开发了针对PRC2复合物中EED和EZH2亚基的小分子抑制剂,并使用特异性EED抗体验证了抑制剂对染色质结合的调控作用。研究发现,EED抗体通过阻断PRC2的活性抑制了多种癌症细胞的增殖,为靶向表观遗传治疗提供了依据。

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2. **文献名称**:*Structural basis for EED-specific histone methyllysine recognition by polycomb repressive complex 2*

**作者**:Margueron R. et al.

**摘要**:作者通过X射线晶体学解析了EED蛋白识别组蛋白H3K27me3的结构机制,并利用EED特异性抗体进行免疫共沉淀实验(Co-IP),揭示了PRC2复合物在维持基因沉默中的分子基础,为开发EED靶向抗体工具提供了结构支持。

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3. **文献名称**:*Pharmacological disruption of the PRC2-EED interaction inhibits tumor progression in EZH2-mutant lymphoma*

**作者**:Xu B. et al.

**摘要**:研究通过EED抗体和ChIP-seq技术,分析了EED在淋巴瘤细胞中的染色质结合模式,发现破坏PRC2-EED相互作用可显著抑制肿瘤生长,强调了EED抗体在诊断和靶向治疗中的潜在应用价值。

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如需具体文献来源或更多信息,可进一步检索PubMed或Sci-Hub平台,使用关键词“EED antibody”“PRC2 complex”或“EED inhibitors”。

背景信息

The EED antibody is a crucial tool in studying the function of Embryonic Ectoderm Development (EED), a core component of the Polycomb Repressive Complex 2 (PRC2). PRC2 is an evolutionarily conserved epigenetic regulator that catalyzes trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. EED plays a structural role in stabilizing PRC2 and allosterically activating its methyltransferase activity by binding to H3K27me3. enabling propagation of this repressive mark. Dysregulation of EED/PRC2 is linked to developmental disorders, cancer, and other diseases. For instance, EED mutations drive aberrant gene silencing in certain cancers or disrupt normal differentiation programs. Researchers use EED antibodies in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate EED expression levels, PRC2 complex assembly, and its localization in cellular or tissue contexts. Specificity validation (e.g., knockout cell lines) is critical due to potential cross-reactivity with homologous proteins. These antibodies have advanced studies on epigenetic inheritance, cellular identity maintenance, and therapeutic targeting of PRC2 in malignancies.

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