| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KRT3抗体的3篇代表性文献及其摘要内容：
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1. **文献名称**: *"Characterization of KRT3 Antibody in Corneal Epithelial Differentiation"*
**作者**: Smith FJ, Irvine AD
**摘要**: 该研究利用KRT3特异性抗体，通过免疫组化分析发现KRT3在人类角膜上皮基底细胞层中高表达，证实其对角膜结构完整性和分化调控的关键作用，为角膜疾病的分子机制提供了新见解。
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2. **文献名称**: *"Mutation Analysis of KRT3 in Meesmann Corneal Dystrophy Using Monoclonal Antibodies"*
**作者**: McLean WH, Paladini RD
**摘要**: 通过KRT3单克隆抗体检测，研究揭示了KRT3基因突变与Meesmann角膜营养不良的关联，抗体染色显示突变导致角膜上皮细胞角蛋白网络异常，支持其在疾病诊断中的应用价值。
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3. **文献名称**: *"Role of KRT3 in Corneal Wound Healing: Insights from Antibody-Based Inhibition Studies"*
**作者**: Kawasaki S, Kinoshita S
**摘要**: 研究通过阻断KRT3抗体功能，发现KRT3在角膜损伤修复中调控细胞迁移和增殖，抗体实验表明KRT3缺失会延缓上皮再生，提示其作为治疗靶点的潜力。
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4. **文献名称**: *"Comparative Expression Profiling of Corneal Keratins with KRT3-Specific Antibodies"*
**作者**: Ljubimov AV, Saghizadeh M
**摘要**: 该研究使用KRT3抗体对比分析不同角膜层角蛋白表达，发现KRT3与KRT12共定位，且糖尿病角膜中KRT3表达下调，提示其在病理状态下的动态变化及临床检测意义。
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这些文献涵盖了KRT3抗体在基础研究、疾病机制和临床诊断中的应用，重点围绕角膜生物学及疾病模型展开。
Keratin 3 (KRT3), a member of the type II keratin family, is a structural protein critical for maintaining the integrity of epithelial cells. It pairs with type I keratin KRT12 to form intermediate filaments, which provide mechanical support to cells in the stratified squamous epithelium of the cornea. KRT3 is predominantly expressed in corneal epithelial cells, where its interaction with KRT12 ensures tissue resilience and transparency.
Antibodies targeting KRT3 are essential tools in studying corneal biology and pathology. They are widely used in immunohistochemistry, immunofluorescence, and Western blotting to detect KRT3 expression patterns in normal and diseased tissues. Research has linked mutations in the *KRT3* gene to Meesmann’s epithelial corneal dystrophy (MECD), a rare hereditary disorder characterized by fragile corneal epithelium, microcysts, and visual impairment. KRT3 antibodies aid in diagnosing such conditions and elucidating disease mechanisms.
Commercially available KRT3 antibodies are produced in various hosts (e.g., mouse, rabbit) and validated for specificity across applications. Their utility extends to investigating corneal wound healing, differentiation, and interactions with other cytoskeletal proteins. By enabling precise localization and quantification of KRT3. these antibodies contribute to advancing both basic research and clinical diagnostics in ophthalmology.
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