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Mouse Monoclonal EED Antibody

  • 中文名: EED抗体
  • 别    名: nan
货号: IPDX15036
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/100-1/200 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesETS2IT1
WB Predicted band size53 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human ETS2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于EED抗体的3篇示例文献(注:以下内容为模拟生成,实际文献需通过学术数据库检索):

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### 1. **"Structural basis for EED-mediated histone methylation recognition by the Polycomb repressive complex 2"**

**作者**: Cao, Q., Zhang, Y.

**摘要**: 本研究通过X射线晶体学解析了EED蛋白与组蛋白H3K27me3修饰的结合机制,揭示了EED在PRC2复合物中识别表观遗传标记的结构基础。研究中使用了特异性EED抗体进行免疫共沉淀(Co-IP),验证了EED与其他PRC2亚基(如SUZ12和EZH2)的相互作用。

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### 2. **"EED inhibition disrupts PRC2 activity and suppresses tumor growth in glioblastoma"**

**作者**: Kim, H., et al.

**摘要**: 该研究通过靶向EED的小分子抑制剂阻断PRC2功能,发现其在胶质母细胞瘤模型中显著抑制肿瘤增殖。实验中使用EED抗体进行Western blot和免疫组化(IHC),证实了EED在肿瘤组织中的高表达及其与H3K27me3水平的相关性。

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### 3. **"Development of a high-affinity monoclonal antibody against EED for epigenetic therapy screening"**

**作者**: Li, S., et al.

**摘要**: 研究团队开发了一种高亲和力的单克隆EED抗体,用于检测PRC2复合物的组装状态及药物筛选。通过ELISA和流式细胞术验证了抗体的特异性,并利用该抗体发现了一类新型EED抑制剂(如EED226),可有效阻断PRC2的甲基转移酶活性。

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### 4. **"EED loss in hematopoietic stem cells leads to aberrant self-renewal and leukemia"**

**作者**: Montgomery, N.D., et al.

**摘要**: 本研究在小鼠模型中敲除EED基因,发现其缺失导致造血干细胞自我更新异常并诱发白血病。通过ChIP-seq(使用EED抗体富集DNA)和RNA-seq分析,揭示了EED通过调控H3K27me3依赖的基因沉默维持正常造血功能的机制。

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如需具体文献,建议通过PubMed或Web of Science检索关键词“EED antibody”、“PRC2 EED”或“EED H3K27me3”获取最新研究。

背景信息

EED (Embryonic Ectoderm Development) is a core component of the Polycomb Repressive Complex 2 (PRC2), a critical epigenetic regulator that mediates gene silencing through trimethylation of histone H3 on lysine 27 (H3K27me3). As a scaffolding protein, EED facilitates PRC2 assembly and allosterically activates its methyltransferase activity. Its unique structure, including a β-propeller domain, enables recognition of H3K27me3 marks, creating a positive feedback loop for PRC2-mediated chromatin repression. EED plays essential roles in embryonic development, cell differentiation, and stem cell maintenance by dynamically regulating transcriptional programs. Dysregulation of EED is implicated in cancers (e.g., lymphomas, gliomas) and overgrowth syndromes like Weaver syndrome. EED-specific antibodies are vital tools for studying PRC2 function, enabling detection of EED expression, localization, and interactions via techniques like Western blot, immunohistochemistry, and co-immunoprecipitation. They also support chromatin immunoprecipitation (ChIP) to map PRC2 occupancy and H3K27me3 distribution. Recent research explores therapeutic targeting of EED-PRC2 in diseases driven by epigenetic dysfunction, highlighting the antibody's importance in both basic research and drug development.

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