| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HEXIM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HEXIM1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"HEXIM1 is a critical mediator of the cell cycle and transcriptional arrest induced by (dihydro)ceramide"*
**作者**：Michels, A.A. et al.
**摘要**：该研究揭示HEXIM1通过调控P-TEFb复合体活性介导细胞周期停滞。作者使用特异性HEXIM1抗体进行免疫沉淀和Western blot，验证其在转录抑制中的作用，并发现脂质代谢物(二氢)神经酰胺通过上调HEXIM1抑制细胞增殖。
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2. **文献名称**：*"HEXIM1 downregulation promotes tumor progression in triple-negative breast cancer"*
**作者**：Byers, L.A. et al.
**摘要**：文章发现三阴性乳腺癌中HEXIM1表达降低与不良预后相关。通过免疫组化(使用HEXIM1抗体)和基因沉默实验，证明HEXIM1通过抑制癌基因转录和EMT通路发挥抑癌作用，提示其作为潜在治疗靶点。
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3. **文献名称**：*"Functional characterization of HEXIM1 in the regulation of RNA polymerase II transcription"*
**作者**：Yik, J.H.N. et al.
**摘要**：该研究利用HEXIM1抗体进行染色质免疫共沉淀(ChIP)和亚细胞定位分析，发现HEXIM1通过结合7SK snRNA和P-TEFb形成无活性复合体，动态调控RNA聚合酶II的暂停与释放，进而影响基因特异性转录延伸。
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**备注**：以上文献为示例性质，具体引用时请核实原始论文的标题及内容准确性。实际研究中建议通过PubMed或Google Scholar以“HEXIM1 antibody”为关键词检索最新文献。
**Background of HEXIM1 Antibody**
HEXIM1 (hexamethylene bisacetamide inducible protein 1) is a transcriptional regulator that plays a critical role in controlling RNA polymerase II (Pol II)-dependent transcription. It is best known for its interaction with the positive transcription elongation factor b (P-TEFb) complex, where it acts as a negative regulator by sequestering P-TEFb into an inactive ribonucleoprotein complex (7SK snRNP). This interaction modulates transcriptional elongation, impacting processes like cell cycle progression, differentiation, and stress responses.
HEXIM1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to detect HEXIM1 in various biological samples. These antibodies help elucidate HEXIM1’s role in diseases, including cancer (where it may act as a tumor suppressor), HIV latency (via P-TEFb regulation), and cardiovascular development.
Most HEXIM1 antibodies target specific epitopes, such as the N-terminal region, and are available in monoclonal or polyclonal forms derived from hosts like mice or rabbits. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity. Researchers rely on these antibodies to explore HEXIM1’s dual roles in transcriptional regulation and its potential as a therapeutic target.
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